Preparation of W1/O/W2 emulsions and droplet size distribution measurements by pulsed-field gradient nuclear magnetic resonance (PFG-NMR) technique
Preparation of W1/O/W2 emulsions and droplet size distribution measurements by pulsed-field gradient nuclear magnetic resonance (PFG-NMR) technique
复制标题
通过脉冲场梯度核磁共振 (PFG-NMR) 技术制备 W1/O/W2 乳液并测量液滴尺寸分布
DOI：
10.1002/ejlt.200800272
复制
发表时间：
2009
影响因子：
2.7
通讯作者：
G. Guthausen
中科院分区：
文献类型：
--
作者：
Frederik Wolf;Lena L. Hecht;H. Schuchmann;E. H. Hardy;G. Guthausen
w1 /O/ w2型的多种乳剂是生命科学的主要研究方向，为水溶性活性剂的包封提供了可能性。在食品科学中，它们也用于减脂。内部和外部乳剂的液滴大小分布对乳剂的流变性和感观性、释放动力学以及结构和微生物稳定性具有重要影响。然而，确定内部和外部液滴大小分布是一个主要的挑战，因为传统的测量技术不能应用。脉冲场梯度核磁共振（PFG-NMR）是众所周知的用于液滴尺寸测定的非破坏性工具，特别是在简单的乳剂中。以聚甘油-聚蓖麻油酸酯（PGPR）和聚氧乙烯-20-山梨醇单月桂酸酯（Tween 20）为乳化剂，采用转子-定子乳化机制备了w1 /O/ w2型双乳。PFG-NMR应用于测量内相（w1）液滴尺寸分布以及表征O相。将w1值与简单乳液（w1 /O型）的激光衍射结果进行了比较，发现如果考虑外水相（w2）的限制扩散和其他影响，则在实验误差范围内是一致的。
Multiple emulsions of W 1 /O/W 2 type are of major interest in life sciences, offering possibilities for the encapsulation of water-soluble active agents. In food science, they are also applied for fat reduction. The droplet size distributions of the inner and outer emulsions are of main importance as they influence the rheological and sensorial properties, the release kinetics, as well as the structural and microbial stability. However, the determination of the inner and outer droplet size distributions is a major challenge, as conventional measurement techniques cannot be applied. Pulsed-field gradient nuclear magnetic resonance (PFG-NMR) is well known as a non-destructive tool for droplet size determination, especially in simple emulsions. In this work, double emulsions of the W 1 /O/W 2 type were prepared with polyglycerol-polyricinoleate (PGPR) and polyoxyethylen-20-sorbitan monolaurate (Tween 20) as emulsifiers by means of rotor-stator emulsification machines. PFG-NMR was applied for measurements of the inner phase (W 1 ) droplet size distribution as well as for the characterization of the O phase. The W 1 values were compared with results from laser light diffraction of simple emulsions (W 1 /O type) and were found to be consistent within the experimental errors, if restricted diffusion in the outer water phase (W 2 ) and additional effects are considered.