Epidermal growth factor-induced topoisomerase(s). Intracellular translocation and relation to DNA synthesis.
Epidermal growth factor-induced topoisomerase(s). Intracellular translocation and relation to DNA synthesis.
复制标题
表皮生长因子诱导的拓扑异构酶。
DOI：
10.1016/0014-4827(83)90323-3
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发表时间：
1983
影响因子：
3.7
通讯作者：
Shimizu,N
中科院分区：
文献类型：
--
作者：
Miskimins,R;Miskimins,WK;Bernstein,H;Shimizu,N
我们利用表皮生长因子(EGF)研究了真核细胞拓扑异构酶与DNA合成的关系。我们发现EGF能刺激人成纤维细胞和瑞士/3T3小鼠成纤维细胞的拓扑异构酶活性。首先出现的是细胞质的增加，然后是细胞核的活动增加。细胞核的增加与DNA合成的增加相对应。一种II型拓扑异构酶被刺激，这表明松弛反应对ATP的依赖和连环链烷的形成。我们还发现，在细胞质中的拓扑异构酶活性是可沉淀的，这表明它要么是膜相关的，要么是超分子复合体。EGF对拓扑异构酶活性的刺激可能是EGF诱导DNA合成和细胞分裂过程中的关键步骤。
We have used epidermal growth factor (EGF) to investigate the relationship between eukaryotic topoisomerases and DNA synthesis. We found that EGF stimulates topoisomerase activity in human fibroblasts and Swiss/3T3 mouse fibroblasts. The first increase is seen in the cytoplasm, followed by increased activity in the nucleus. The nuclear increases correspond to increases in DNA synthesis. A type II topoisomerase is stimulated as indicated by the ATP dependence of the relaxing reaction and by the formation of catenanes. We have also found that the topoisomerase activity in the cytoplasm is sedimentable indicating that it is either membrane-associated or in a supramolecular complex. The stimulation of topoisomerase activity by EGF may represent a key step in the process by which EGF induces DNA synthesis and cell division.