Developmental changes in the basement membrane of the normal and hypothyroid postnatal rat testis: segmental localization of fibulin-2 and fibronectin.
Developmental changes in the basement membrane of the normal and hypothyroid postnatal rat testis: segmental localization of fibulin-2 and fibronectin.
复制标题
正常和甲状腺功能减退的产后大鼠睾丸基底膜的发育变化：fibulin-2 和纤连蛋白的节段定位。
DOI：
10.1095/biolreprod58.5.1123
复制
发表时间：
1998
影响因子：
3.6
通讯作者：
Dziadek,M
中科院分区：
文献类型：
--
作者：
Loveland,K;Schlatt,S;Sasaki,T;Chu,ML;Timpl,R;Dziadek,M
为了表征生后大鼠睾丸细胞外基质的发育变化与生殖细胞成熟时间的关系，我们对正常和丙基硫氧嘧啶(PTU)诱导的甲状腺功能低下动物的睾丸中的纤维蛋白-1、纤维蛋白-2和其他基质成分进行了免疫定位。与生后发育过程中存在于生精小管基底膜(BM)中的层粘连蛋白、尼多芬和Perlecan不同，纤溶蛋白从生后小管基底膜中消失。Fiblin-1在第5天后不再被检测到，而Fiblin-2在第10天和第15天开始以节段性的方式定位于每个生精小管的BM内，并在第20天消失。FN在第10天和第15天在每个生精小管的BM免疫染色水平上显示出节段性的模式，在早期和晚期可见更均匀的染色。VI型胶原最初局限于第5天睾丸的间质基质，并在后期逐渐与生精小管BM联系得更加紧密。PTU组大鼠睾丸纤维蛋白-2的消失和VI型胶原的BM结合均延迟。纤维蛋白-2和纤维连接蛋白染色模式的发育变化与肾小管周细胞在内曲细精管BM上的黏附和排列相一致。PTU处理的大鼠生精管BM成熟延迟，表明BM成分的变化与睾丸细胞发育有关。
To characterize developmental changes in the extracellular matrix of the postnatal rat testis in relationship to the timing of germ cell maturation, we immunolocalized fibulin-1, fibulin-2, and other matrix components in the testes of normal and propylthiouracil (PTU)-induced hypothyroid animals. Unlike laminin, nidogen, and perlecan, which were present in the seminiferous tubule basement membrane (BM) throughout postnatal development, fibulins were found to disappear from the postnatal tubule BM. Fibulin-1 was no longer detected after Day 5 whereas fibulin-2 became localized in a segmental manner within the BM of each seminiferous tubule on Days 10 and 15 and disappeared by Day 20. Fibronectin showed a segmental pattern in the level of immunostaining of the tubule BM on Days 10 and 15, with a more uniform staining seen at earlier and later ages. Collagen VI was initially confined to the interstitial matrix in the Day 5 testis and became progressively more closely associated with the seminiferous tubule BM at later stages. The disappearance of fibulin-2 and the BM-association of collagen VI were both delayed in the PTU-treated testes. The developmental changes in the staining patterns for fibulin-2 and fibronectin coincide with the adhesion and alignment of peritubular cells on the inner seminiferous tubule BM. The delay in maturation of the seminiferous tubule BM in the testes of PTU-treated rats demonstrates a correlation between changes in the composition of the tubule BM and cellular development of the testis.