Biosynthesis of endogenous cardiac glycosides by mammalian adrenocortical cells: three steps forward.
Biosynthesis of endogenous cardiac glycosides by mammalian adrenocortical cells: three steps forward.
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哺乳动物肾上腺皮质细胞生物合成内源性强心苷：前进三步。
作者：
J. Hamlyn
2003年是分离和鉴定矿物皮质激素醛固酮50周年，这是一个重大事件，因为醛固酮被广泛认为是哺乳动物肾上腺中最后一种主要的代谢活性类固醇(1)。在本期杂志中，Qazzaz等人(2)描述了支持在肾上腺中重新合成一种材料的可能性的研究，这种材料的整体物理化学、免疫交叉反应和色谱特征与心糖苷（CG）地高辛及其二氢类似物（内酯环完全饱和）没有区别。利用从肾上腺皮质细胞中提取的小鼠肿瘤细胞系，研究人员发现，醋酸中的14C标签和胆固醇中的14C标签可以结合到两种地高辛样物质中。此外，用美伐他汀抑制羟甲基戊二酰(HMG)-辅酶a还原酶减少了两种产物的14C标记，同时没有解决侧链切割的问题。虽然在研究中没有使用复杂的分析方法，但内源性产物的指纹图谱和14C标记的行为完全符合地高辛和二氢地高辛或其各自异构体的存在，因此，与…
2003 was the 50th anniversary of the isolation and identification of the mineralocorticoid aldosterone—a momentous event if only because aldosterone has been widely regarded as the last major metabolically active steroid to be described in the mammalian adrenal gland (1). In this issue of the journal, Qazzaz et al. (2) describe studies that support the possibility of de novo biosynthesis, in the adrenal, of a material whose overall physicochemical, immunocrossreactive, and chromatographic characteristics are indistinguishable from those of the cardiac glycoside (CG) digoxin and its dihydro analog (in which the lactone ring is fully saturated). Using a mouse tumor cell line derived from adrenocortical cells, the authors show that the 14C label from acetic acid, as well as that from labeled cholesterol, becomes incorporated into the two digoxin-like materials. Moreover, inhibition of hydroxymethylglutaryl (HMG)-CoA reductase with Mevastatin reduced the 14C labeling of both products while leaving the issue of side chain cleavage unanswered. Although sophisticated analytical methods were not used in the study, the fingerprinting of the endogenous products and the behavior of the 14C labeling are entirely consistent with the presence of digoxin and dihydrodigoxin or their respective isomers and, therefore, with …
影响因子：
4.8
作者：
J. Laredo;B. Hamilton;J. Hamlyn
通讯作者：
J. Laredo;B. Hamilton;J. Hamlyn
哺乳动物洋地黄样因子的从头生物合成和放射性标记。
影响因子：
9.3
作者：
Qazzaz,HassanMAM;Cao,Zhimin;Bolanowski,DuaneD;Clark,BarbaraJ;ValdesJr,Roland
通讯作者：
ValdesJr,Roland