The number of single nucleotide polymorphisms and on-farm data required for whole-herd parentage testing in dairy cattle herds
The number of single nucleotide polymorphisms and on-farm data required for whole-herd parentage testing in dairy cattle herds
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DOI：
10.3168/jds.2008-1086
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发表时间：
2009-01-01
影响因子：
3.5
通讯作者：
Spelman, R. J.
中科院分区：
文献类型：
--
作者：
Fisher, P. J.;Malthus, B.;Spelman, R. J.
利用单核苷酸多态性（SNP）的新平台比传统的基于微卫星的平台具有操作优势，使其成为亲子排除的有希望的替代方案。通过模拟和经验数据，40-SNP小组（其中次要等位基因频率平均为0.35）被证明是与当前用于亲子鉴定的14-微卫星小组相当或更好的诊断工具新西兰奶牛。单独的40个SNP没有足够的排除能力将超过75%的后代与正确的父系和母系匹配。利用交配记录和分组的后代和母鼠的出生和产犊日期，分别减少了公畜-母畜组合的数量，每个后代进行了测试，并显着增加了SNP的效用。这些结果强调了将基因型与农场数据相结合的重要性，以最大限度地提高在新西兰奶牛群中分配亲子关系的能力。
New platforms utilizing single nucleotide polymorphisms (SNP) offer operational advantages over the conventional microsatellite-based ones, making them a promising alternative for parentage exclusion. Through simulation and empirical data, a 40-SNP panel (where the minor allele frequency was 0.35 on average) was shown to be a comparable or better diagnostic tool than the current 14-microsatellite panel that is used to parentage test New Zealand dairy animals. The 40 SNP alone did not have sufficient power of exclusion to match more than 75% of the progeny to the correct sire and dam. Utilizing mating records and grouping progeny and dams by birth and calving dates, respectively, decreased the number of sire-dam combinations that each progeny was tested against and dramatically increased the utility of the SNP. These results highlight the importance of combining genotypes with on-farm data to maximize the ability to assign parentage in the New Zealand dairy herd.