The knockout of urea transporter-B improves the hemorheological properties of erythrocyte
The knockout of urea transporter-B improves the hemorheological properties of erythrocyte
复制标题
尿素转运蛋白-B的敲除改善了红细胞的血液流变学特性。
DOI：
10.3233/ch-16174
复制
发表时间：
2017-01-01
影响因子：
2.1
通讯作者：
Yang, Baoxue
中科院分区：
文献类型：
--
作者：
Geng, Xiaoqiang;Lei, Tianluo;Yang, Baoxue
目的：尿素转运蛋白B（UT-B）在红细胞中高度表达，对尿素、尿素类似物和水具有特异性的通透性。本研究的目的是使用一系列的生物物理techniques.Methods和Results测定UT-B基因敲除小鼠和野生型小鼠的血液流变学特性。用UT-B抑制剂PU-14体外处理野生型小鼠红细胞，分析处理后红细胞的变形性。结果表明，UT-B基因敲除可改善红细胞的血液流变学特性，包括红细胞变形指数、小变形指数、取向指数、低渗透脆性和高电泳率。UT-B抑制剂PU-14对变形指数的影响与UT-B敲除剂相似。UT-B基因敲除小鼠的全血粘度较野生型小鼠有降低趋势。结论：UT-B基因参与了血液流变学的调节，提示UT-B基因可能成为改善某些代谢性和遗传性疾病血液流变学的潜在治疗靶点。
OBJECTIVE: Urea transporter-B (UT-B), highly expressed in erythrocyte, confers specific permeability to urea, urea analogues and water. The purpose of this study was to determine the hemorheological properties of UT-B null erythrocyte using a series of biophysical techniques.METHODS AND RESULTS: The blood was taken from UT-B knockout and wild-type mice and the hemorheological parameters were measured. The UT-B inhibitor, PU-14, was used to treat the erythrocyte of wild-type mice in vitro and the deformability of the treated erythrocyte was analyzed. The results showed that UT-B knockout improves the hemorheological properties of erythrocyte, including increased erythrocyte deformation index, small deformation index, orientation index, low osmotic fragility and high electrophoretic rate. The UT-B inhibitor PU-14 had the similar effect as UT-B knockout on the deformation indices. The whole blood viscosity in UT-B knockout mice showed reduction trend as compared to wild-type mice.CONCLUSIONS: The data indicate that UT-B is involved in the regulation of hemorheology, which suggests that UT-B may be a potential therapeutic target for improving the hemorheology in some metabolic and hereditary diseases.