Circulating cell-derived microparticles in patients with minimally symptomatic obstructive sleep apnoea
Circulating cell-derived microparticles in patients with minimally symptomatic obstructive sleep apnoea
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DOI：
10.1183/09031936.00107408
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发表时间：
2009-03-01
影响因子：
24.3
通讯作者：
Kohler, M.
中科院分区：
文献类型：
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作者：
Ayers, L.;Ferry, B.;Kohler, M.
中重度阻塞性睡眠呼吸暂停（OSA）与几种促动脉粥样硬化机制和心血管风险增加有关，但尚不清楚轻度症状性OSA是否有类似的影响。循环细胞来源的微粒已被证明具有促炎、促凝和内皮功能损害作用，并可预测亚临床动脉粥样硬化和心血管风险。在57例轻度症状性OSA患者和15例密切匹配的无OSA对照者中，用流式细胞术测量了annexv阳性、血小板、白细胞和内皮细胞来源的微颗粒。在OSA患者中，annexinv阳性微粒的中位（四分位数范围）水平与对照组相比显著升高：分别为2,586 (1,566-3,964)μ L-1和1,206 (474-2,501)μ L-1。与对照组(分别为925 （328- 2068)μ L-1和15 (5-23)μ L-1）相比，OSA患者的血小板源性和白细胞源性微粒水平也显著升高(分别为2267 （1102 - 3592)μ L-1和20 (14-31)μ L-1）。OSA患者的内皮细胞源性微粒水平与对照组相似(13 （8-25)mu L-1与11 (6-17)mu L-1）。在症状轻微的阻塞性睡眠呼吸暂停患者中，与没有阻塞性睡眠呼吸暂停的密切匹配的对照组相比，annexinv阳性、血小板和白细胞来源的微粒水平升高。这些发现表明，这些患者尽管症状轻微，但心血管风险可能增加。
Moderate-severe obstructive sleep apnoea (OSA) has been associated with several pro-atherogenic mechanisms and increased cardiovascular risk, but it is not known if minimally symptomatic OSA has similar effects. Circulating cell-derived microparticles have been shown to have pro-inflammatory, pro-coagulant and endothelial function-impairing effects, as well as to predict subclinical atherosclerosis and cardiovascular risk.In 57 patients with minimally symptomatic OSA, and 15 closely matched control subjects without OSA, AnnexinV-positive, platelet-, leukocyte- and endothelial cell-derived microparticles were measured by flow cytometry.In patients with OSA, median (interquartile range) levels of AnnexinV-positive microparticles were significantly elevated compared with control subjects: 2,586 (1,566-3,964) mu L-1 versus 1,206 (474-2,501) mu L-1, respectively. Levels of platelet-derived and leukocyte-derived microparticles were also significantly higher in patients with OSA (2,267 (1,102-3,592) mu L-1 and 20 (14-31) mu L-1, respectively) compared with control subjects (925 (328-2,068) mu L-1 and 15 (5-23) mu L-1, respectively). Endothelial cell-derived microparticle levels were similar in patients with OSA compared with control subjects (13 (8-25) mu L-1 versus 11 (6-17) mu L-1).In patients with minimally symptomatic obstructive sleep apnoea, levels of AnnexinV-positive, platelet- and leukocyte-derived microparticles are elevated when compared with closely matched control subjects without obstructive sleep apnoea. These findings suggest that these patients may be at increased cardiovascular risk, despite being minimally symptomatic.