Cadmium stress alters cytosine methylation status and expression of a select set of genes in Nicotiana benthamiana
Cadmium stress alters cytosine methylation status and expression of a select set of genes in Nicotiana benthamiana
复制标题
镉胁迫改变本塞姆氏烟草中胞嘧啶甲基化状态和一组选定基因的表达
DOI：
10.1016/j.plantsci.2019.03.021
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发表时间：
2019
期刊：
影响因子：
5.2
通讯作者：
Guo Jiangbo
中科院分区：
文献类型：
--
作者：
Xin Cuihua;Chi Junling;Zhao Yibo;He Yindi;Guo Jiangbo
本文以模式植物本氏烟草（Nicotianabenthamiana）为材料，采用甲基化敏感扩增多态性（MSAP）技术，研究了镉对植物的遗传毒性。在检测的255个位点中，发现14个基因在镉胁迫下表现出改变的胞嘧啶甲基化模式。其中四个基因（NbMORC 3、NbHGS NAT、NbMUT和NbBG）由于其在植物发育中的预测作用而被选择用于进一步分析。镉诱导的胞嘧啶甲基化状态的变化在MSAP片段的选定的基因进行了确认，使用亚硫酸氢盐测序聚合酶链反应（BSP）。此外，这些基因的表达水平被发现与镉剂量相关，这四个基因通过病毒诱导的基因沉默（VIGS）敲低导致异常发育和镉胁迫的敏感性升高。这四个基因的沉默导致了实验植物不同部位镉积累的改变。我们的数据表明，镉暴露引起植物基因组的胞嘧啶甲基化状态的显着变化，从而影响许多基因的表达，这些基因对植物生长至关重要，并参与镉胁迫反应。
In this paper, we evaluated the genotoxicity of cadmium (Cd) in plants by performing a methylation-sensitive amplification polymorphism (MSAP) on the model plantNicotiana benthamiana. Among 255 loci examined, 14 genes were found to show altered cytosine methylation patterns in response to Cd stress. Four of those genes (NbMORC3,NbHGSNAT,NbMUT, andNbBG) were selected for further analysis due to their predicted roles in plant development. Cd-induced changes of cytosine methylation status in MSAP fragments of selected genes were confirmed using bisulfite sequencing polymerase chain reaction (BSP). In addition, the expression levels of these genes were found to correlate with cadmium dosage, and a knock-down of these four genes via virus-induced genes silencing (VIGS) led to abnormal development and elevated sensitivity to cadmium stress. Silencing of these four genes resulted in altered cadmium accumulation in different parts of the experimental plants. Our data indicate that cadmium exposure causes dramatic changes in the cytosine methylation status of the plant genome, thus affecting the expression of many genes that are vital for plant growth and are involved in cadmium stress response.