Sleep apnea is associated with the increase of certain genera of Ruminococcaceae and Lachnospiraceae in the gut microbiome of hypertensive patients
Sleep apnea is associated with the increase of certain genera of Ruminococcaceae and Lachnospiraceae in the gut microbiome of hypertensive patients
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DOI：
10.1080/17476348.2022.2147509
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发表时间：
2022-11
影响因子：
3.9
通讯作者：
Cheng Zhang;Fengwei Chen;Yane Shen;Yuqing Chen;Jing Ma
中科院分区：
文献类型：
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作者：
Cheng Zhang;Fengwei Chen;Yane Shen;Yuqing Chen;Jing Ma
摘要背景阻塞性睡眠呼吸暂停（OSA）和高血压是与肠道生态失调相关的疾病。本研究旨在研究OSA对高血压背景下肠道微生物组的影响，反之亦然。研究设计和方法211例连续筛选的患者中，52例完成了多导睡眠图研究，病史问卷调查和粪便样本收集。对粪便样品进行16 S rRNA基因测序，并使用生物信息学分析多样性、丰富度和微生物类群。结果α多样性在OSA组和高血压组均略有降低，但差异无统计学意义; OSA组缺氧负荷指数（HBI）与Chao 1指数呈弱正相关（r = 0.342，p < 0.05）。有OSA的患者厚壁菌与拟杆菌的比例高于无OSA的患者。在高血压患者中，OSA患者的瘤胃球菌_1、毛梭菌、毛螺菌、[瘤胃球菌]_扭矩_组和未鉴定的毛螺菌科水平高于无OSA患者。相反，在OSA患者中，高血压患者具有较低的粪杆菌和毛螺菌_NK4A136_组水平。结论睡眠呼吸暂停患者肠道菌群的改变可能是睡眠呼吸暂停病理生理的代偿机制。HBI和α多样性之间的正相关性，以及OSA患者中瘤胃球菌科和毛螺菌科某些属的增加可能代表对缺氧的适应性反应。
ABSTRACT Background Obstructive sleep apnea (OSA) and hypertension are interrelated diseases linked to gut dysbiosis. This study aimed to investigate the effect of OSA on the gut microbiome in the context of hypertension and vice versa. Research design and methods Of 211 consecutively screened patients, 52 completed polysomnography study, medical history questionnaires, and fecal sample collection. 16S rRNA gene sequencing was performed on fecal samples, and diversity, richness, and microbial taxa were analyzed using bioinformatics. Results Alpha diversity showed slightly decreased diversity in OSA and hypertension groups without significant difference, and the hypoxia burden index (HBI) showed a weak positive correlation with Chao1 index (r = 0.342, p < 0.05) in OSA patients. Firmicutes-to-Bacteroidetes ratio was higher in patients with than without OSA. In hypertensive patients, those with OSA had higher Ruminococcus_1, Lachnoclostridium, Lachnospira, [Ruminococcus]_torques_group, and unidentified Lachnospiraceae levels than those without OSA. Conversely, in OSA patients, hypertensive patients had lower Faecalibacterium and Lachnospiraceae_NK4A136_group levels. Conclusion The present study suggests a possible compensatory mechanism for gut microbiome changes in sleep apnea pathophysiology. The positive correlation between HBI and alpha diversity, and increase in certain genera of Ruminococcaceae and Lachnospiraceae in OSA patients may represent an adaptive response to hypoxia.