OXA-23-producing Acinetobacter baumannii: a new hotspot of diversity in Rio de Janeiro?
OXA-23-producing Acinetobacter baumannii: a new hotspot of diversity in Rio de Janeiro?
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DOI：
10.1093/jac/dkq406
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发表时间：
2011-01-01
影响因子：
5.2
通讯作者：
Peixe, Luisa
中科院分区：
文献类型：
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作者：
Grosso, Filipa;Carvalho, Karyne Rangel;Peixe, Luisa
本研究对巴西里约热内卢产OXA-23鲍曼不动杆菌临床分离株的群体结构进行了分析，包括PFGE、两种多位点序列分型（MLST）方案、序列组（SG）测定和bla（OXA-51-like）测序。用I-CeuI杂交和PCR方法对bla（OXA-23）基因的基因组背景进行了分析，得到了4个谱系的一致聚类。相应地，用MLST-OD方案（与牛津数据库相关）获得了四种新的序列类型（ST）（ST 131、ST 132、ST 133和ST 134），用MLST-IP方案（由巴斯德研究所开发）获得了四种（ST 79、ST 15和两种新的等位基因谱）。四个SG（SG 1，SG 4和两个新的配置文件）进行了鉴定，允许70%的分离株与欧洲克隆II的关联。bla（OXA-51样）测序显示存在bla（OXA-66）、bla（OXA-69）、bla（OXA-95）和bla（OXA-132）。
This study focused on the population structure of OXA-23-producing Acinetobacter baumannii clinical isolates from Rio de Janeiro, Brazil.The analysis included several genomic typing methods, including PFGE, two multilocus sequence typing (MLST) schemes, sequence group (SG) determination and bla(OXA-51-like) sequencing. The genomic context of the bla(OXA-23) gene was also evaluated using I-CeuI hybridizations and PCR assays.Congruent clustering was obtained revealing four lineages. In accordance, four new sequence types (STs) (ST131, ST132, ST133 and ST134) were obtained with the MLST-OD scheme (associated with the Oxford Database) and four (ST79, ST15 and two new allelic profiles) with the MLST-IP scheme (developed by the Institute Pasteur). Four SGs (SG1, SG4 and two new profiles) were identified, allowing the association of 70% of the isolates with European clone II. bla(OXA-51-like) sequencing revealed the presence of bla(OXA-66), bla(OXA-69), bla(OXA-95) and bla(OXA-132).Identification of new STs together with new SG profiles are findings suggestive of a local diversity hotspot that is worth exploring.