Developmental pattern of Ginkgo biloba levopimaradiene synthase (GbLPS) as probed by promoter analysis in Arabidopsis thaliana
Developmental pattern of Ginkgo biloba levopimaradiene synthase (GbLPS) as probed by promoter analysis in Arabidopsis thaliana
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DOI：
10.1007/s00299-012-1232-1
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发表时间：
2012-06-01
影响因子：
6.2
通讯作者：
Kim, Soo-Un
中科院分区：
文献类型：
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作者：
Kim, Jin-Hee;Lee, Kwang-Il;Kim, Soo-Un
银杏左旋海松二烯合酶（GbLPS）催化银杏内酯生物合成的第一个关键步骤，将牛儿基牛儿基二磷酸转化为左旋海松二烯，然后经过复杂的氧化和碳骨架重排，最终形成银杏内酯。为了研究GbLPS表达的器官特异性和发育特性，研究了GbLPS启动子驱动的GUS在转基因拟南芥中的表达。对转基因拟南芥植株的组织学分析表明，GUS基因主要集中在叶片的表皮、枝条的韧皮部、花的子房和雄蕊以及根的维管系统中。这些观察结果与根和雄球花中LPS转录本的发生相关。茉莉酸甲酯处理后，根中的报告基因表达显著上调，而叶和花中的表达变化不大。目前的研究结果支持银杏内酯的生物合成在银杏植物的根，并建议通过韧皮部转运发生。
Levopimaradiene synthase (GbLPS) of Ginkgo biloba catalyzes the first committed step in ginkgolide biosynthesis by converting geranylgeranyl diphosphate into levopimaradiene, which subsequently undergoes complex oxidation step and rearrangement of carbon skeleton, leading to formation of ginkgolides. To assess the organ-specificity and developmental characteristics of GbLPS expression, the GbLPS promoter-driven GUS expression in transgenic Arabidopsis was studied. Histological analysis of the transgenic Arabidopsis plant showed that the GUS accumulation was mainly localized in the epidermis of leaves, phloem of the shoots, ovaries and stamens of flowers, and vasculature of roots. These observations correlate with the occurrence of LPS transcripts in roots and male strobili of . Treatment of methyl jasmonate on the transformant exhibited significant upregulation of the reporter gene in the roots with little change in leaves and flowers. The present findings support biosynthesis of ginkgolide in the roots of Ginkgo plant and suggest translocation occurs through the phloem.