Immunohistochemical assessment of estrogen receptor distribution in the human endometrium throughout the menstrual cycle.
Immunohistochemical assessment of estrogen receptor distribution in the human endometrium throughout the menstrual cycle.
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DOI：
10.1097/00006254-198510000-00017
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发表时间：
1984-11
期刊：
影响因子：
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通讯作者：
M. Press;N. Nousek-Goebl;W. King;A. Herbst;G. Greene
中科院分区：
文献类型：
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作者：
M. Press;N. Nousek-Goebl;W. King;A. Herbst;G. Greene
用抗雌激素受体单克隆抗体（H226Sp γ和H222Sp γ）与间接免疫过氧化物酶技术定位正常人子宫内膜冰冻切片中的雌激素受体。雌激素受体在绝大多数的上皮细胞和基质细胞的细胞核中检测到所有早期，中期和晚期增殖阶段的卵巢癌。然而，中期和晚期分泌期雌激素受体的本地化的量显着减少。雌激素受体在分泌期中、晚期卵巢功能区的特异性染色较弱，仅限于散在的上皮细胞和间质细胞核。相反，在基底部的一些腺体的上皮细胞核中观察到受体的强染色。早期分泌期似乎是一个过渡期，从强和无处不在的染色的受体特征的增殖期的雌激素受体的定位特征的功能从中期和晚期分泌的雌激素受体的弱，局灶性的模式。绝经后卵巢上皮细胞和间质细胞雌激素受体的核定位一致。子宫肌层平滑肌细胞核中也含有雌激素受体。子宫血管内皮细胞未见受体定位。雌激素受体的特异性染色总是局限于细胞核，没有观察到特异性细胞质染色。
Monoclonal antiestrophilin antibodies (H226Sp gamma and H222Sp gamma) were used with an indirect immunoperoxidase technique to localize estrogen receptor in frozen sections of normal human endometrium. Estrogen receptor was detected in the nuclei of the vast majority of epithelial and stromal cells from all early, middle, and late proliferative phase endometria. However, middle and late secretory phase endometria showed a dramatic reduction in the amount of estrogen receptor localized. Specific staining for estrogen receptor in the functionalis of middle and late secretory phase endometria was weak and limited to nuclei of scattered epithelial and stromal cells. In contrast, strong staining for receptor was observed in epithelial cell nuclei of some glands in the basalis. The early secretory phase appeared to be a period of transition from the strong and ubiquitous staining for receptor characteristic of proliferative phase endometria to the weak, focal pattern of estrogen receptor localization characteristic of the functionalis from middle and late secretory endometria. Postmenopausal endometria showed consistently strong nuclear localization of estrogen receptor in epithelial and stromal cells. Myometrial smooth muscle cell nuclei also contained estrogen receptor. However, the endothelial cells of uterine vessels showed no localization of receptor. Specific staining for estrogen receptor was always limited to nuclei; no specific cytoplasmic staining was observed.