Karyotyping mouse cells.
Karyotyping mouse cells.
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DOI：
10.1101/pdb.prot4706
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发表时间：
2008-05-01
期刊：
影响因子：
--
通讯作者：
Behringer, Richard
中科院分区：
文献类型：
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作者：
Nagy, Andras;Gertsenstein, Marina;Behringer, Richard
现在，大多数小鼠染色体带的制备都是通过风干来完成的，本质上，需要生产细胞悬浮液作为起点。一些样本，如血液培养物、腹水或悬浮细胞，将已经处于悬浮状态；其他细胞，如骨髓、实体瘤或作为附着层生长的细胞，必须转化为悬浮液。下面概述了胚胎癌细胞核型和带型的基本步骤。
INTRODUCTIONThe majority of mouse chromosome preparations for banding are now made by air-drying and, in essence, require the production of a cell suspension as a starting point. Some samples such as blood cultures, ascitic fluids, or cells growing in suspension will already be in suspension; others, such as bone marrow, solid tumors, or cells growing as attached layers in culture must be converted to suspensions. The basic steps in karyotyping and banding embryonal carcinoma cells are outlined below.