Identification and Characterization of a Chitinase-Produced Bacillus Showing Significant Antifungal Activity
Identification and Characterization of a Chitinase-Produced Bacillus Showing Significant Antifungal Activity
复制标题
具有显着抗真菌活性的几丁质酶产生的芽孢杆菌的鉴定和表征
DOI：
10.1007/s00284-009-9363-5
复制
发表时间：
2009-05-01
影响因子：
2.6
通讯作者：
Chen, Yue-Hua
中科院分区：
文献类型：
--
作者：
Xiao, Liang;Xie, Chi-chu;Chen, Yue-Hua
菌株MY 75是一株革兰氏阳性好氧内生孢子形成菌，在几丁质粉末诱导下，能分泌高水平的胞外几丁质酶（4.645U/ml）。利用Biolog MicroLog微生物鉴定系统和16 S rDNA、gyrA和rpoB基因序列分析，将该菌株鉴定为地衣芽孢杆菌（Bacillus licheniformis）。菌株MY 75对两种主要农业病原真菌赤霉病菌和尼日尔曲霉的生长具有抑制作用，并能完全抑制其孢子萌发。几丁质酶在该菌株的抑菌活性中起重要作用。
Strain MY75 is a gram-positive, aerobic, endospore-forming bacterium that can secrete high levels of extracellular chitinase (4.645 U/ml) when chitin powder exists as an inducer. This strain was identified as Bacillus licheniformis using the Biolog MicroLog microbial identification system and sequence analysis of 16S rDNA, gyrA and rpoB genes. Strain MY75 has the ability to inhibit the growth of Gibberella saubinetii and Aspergillus niger, two major pathogenic fungi in agriculture, and to restrain their spore germination completely. The chitinase was proved to play an important role in the strain's antifungal activity.