Compact and flexible raster scanning multiphoton endoscope capable of imaging unstained tissue
Compact and flexible raster scanning multiphoton endoscope capable of imaging unstained tissue
复制标题
DOI：
10.1073/pnas.1114746108
复制
发表时间：
2011-10-25
影响因子：
11.1
通讯作者：
Xu, Chris
中科院分区：
文献类型：
--
作者：
Rivera, David R.;Brown, Christopher M.;Xu, Chris
我们提出了一种紧凑和灵活的内窥镜（3毫米外径，4厘米刚性长度），利用一个小型化的谐振/非谐振光纤光栅扫描仪和多元素梯度指数透镜组件的双光子激发的内源性荧光和二次谐波产生生物组织成像。小型化光栅扫描器是通过将商用双包层光纤（DCF）安装到两个压电双晶片上来制造的，这两个压电双晶片被对准，使得它们的弯曲轴彼此垂直。激光照明的快速横向扫描在4.1帧/秒（每帧512线）是通过同时驱动DCF悬臂在其谐振频率在一个维度和非谐振在正交轴。DCF在扫描仪中的实现使得能够同时递送飞秒脉冲800 nm激发源和信号的落射收集。我们的设备是能够实现一个视野（FOVxy）的110 μ m的110 μ m的高度均匀的像素停留时间。双光子成像的横向和轴向分辨率分别为0.8和10 μ m。内窥镜的成像能力通过在不需要染色的情况下通过收集固有荧光和二次谐波信号对离体小鼠组织进行成像来证明。这里呈现的结果表明，我们的设备可以在未来应用于进行微创体内光学活检的医疗诊断。
We present a compact and flexible endoscope (3-mm outer diameter, 4-cm rigid length) that utilizes a miniaturized resonant/nonresonant fiber raster scanner and a multielement gradient-index lens assembly for two-photon excited intrinsic fluorescence and second-harmonic generation imaging of biological tissues. The miniaturized raster scanner is fabricated by mounting a commercial double-clad optical fiber (DCF) onto two piezo bimorphs that are aligned such that their bending axes are perpendicular to each other. Fast lateral scanning of the laser illumination at 4.1 frames/s (512 lines per frame) is achieved by simultaneously driving the DCF cantilever at its resonant frequency in one dimension and nonresonantly in the orthogonal axis. The implementation of a DCF into the scanner enables simultaneous delivery of the femtosecond pulsed 800-nm excitation source and epi-collection of the signal. Our device is able to achieve a field-of-view (FOVxy) of 110 mu m by 110 mu m with a highly uniform pixel dwell time. The lateral and axial resolutions for two-photon imaging are 0.8 and 10 mu m, respectively. The endoscope's imaging capabilities were demonstrated by imaging ex vivo mouse tissue through the collection of intrinsic fluorescence and second-harmonic signal without the need for staining. The results presented here indicate that our device can be applied in the future to perform minimally invasive in vivo optical biopsies for medical diagnostics.