Contrasting localization of c-Myc with other Myc superfamily transcription factors in the human hair follicle and during the hair growth cycle
Contrasting localization of c-Myc with other Myc superfamily transcription factors in the human hair follicle and during the hair growth cycle
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DOI：
10.1046/j.1523-1747.2001.12771234.x
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发表时间：
2001-04-01
影响因子：
6.5
通讯作者：
Philpott, MP
中科院分区：
文献类型：
--
作者：
Bull, JJ;Müller-Röver, S;Philpott, MP
哺乳动物毛囊是一个高度动态的皮肤附件，经历重复的生长和退化周期，涉及细胞增殖，分化和凋亡的密切协调调节。Myc转录因子超家族与许多组织中这些过程的调节密切相关。使用免疫组织化学，我们已经调查了c-Myc，N-Myc，最大，和Mad 1 -4在人类头发生长周期的不同阶段的表达模式。N-Myc，最大值，Mad 1，Mad 3免疫反应检测在表皮和上皮细胞的生长期和休止期毛囊。毛囊c-Myc免疫反应性的三种不同的模式进行了观察。在漏斗部，c-Myc染色主要是在基底层，在终末分化的基底层上几乎没有可检测的免疫反应性，这种模式是类似的，在表皮中看到的，相反，c-Myc在毛囊球中的表达被发现在增殖的生发上皮细胞和终末分化的基质细胞，产生的毛纤维。最后，强烈的c-Myc免疫反应检测外根鞘的隆起区域。使用C8/144 B抗体作为凸起标记物，我们证实了外根鞘中的c-Myc免疫反应性与假定的毛囊干细胞室相关。c-Myc在隆突中的表达与毛发生长周期阶段无关。我们的数据表明，Myc超家族成员在毛囊的不同上皮隔室中发挥不同的功能，并可能在确定假定的干细胞隔室中的细胞命运中发挥重要作用。
The mammalian hair follicle is a highly dynamic skin appendage that undergoes repeated cycles of growth and regression, involving closely co-ordinated regulation of cell proliferation, differentiation, and apoptosis. The Myc superfamily of transcription factors have been strongly implicated in the regulation of thee processes in many tissues. Using immunohistochemistry, we have investigated the patterns of c-Myc, N-Myc, Max, and Mad1-4 expression at different stages of the human hair growth cycle. N-Myc, Max, Mad1, and Mad3 immunoreactivity was detected in the epidermis and the epithelium of both anagen and telogen hair follicles. Three distinct patterns of hair follicle c-Myc immunoreactivity were observed. In the infundibulum, c-Myc staining was predominantly in the basal layers, with little detectable immunoreactivity in the terminally differentiating suprabasal layers; this pattern was similar to that seen in the epidermis, In contrast, c-Myc expression in the follicle bulb was found both in the proliferating germinative epithelial cells and in the terminally differentiating matrix cells that give rise to the hair fiber. Finally, intense c-Myc immunoreactivity was detected in the bulge region of the outer root sheath. Using the C8/144B antibody as a bulge marker, we confirmed that c-Myc immunoreactivity in the outer root sheath correlates with the putative hair follicle stem cell compartment. c-Myc expression in the bulge was independent of the hair growth cycle stage. Our data suggest that Myc superfamily members serve different functions in separate epithelial compartments of the hair follicle and may play an important role in determining cell fate within the putative stem cell compartment.