Epstein-Barr virus can establish infection in the absence of a classical memory B-cell population
Epstein-Barr virus can establish infection in the absence of a classical memory B-cell population
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DOI：
10.1128/jvi.79.17.11128-11134.2005
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发表时间：
2005-09-01
影响因子：
5.4
通讯作者：
Crawford, DH
中科院分区：
文献类型：
--
作者：
Conacher, M;Callard, R;Crawford, DH
EB病毒(Epstein-Barr Virus，EBV)是一种普遍存在的人类疱疹病毒，在初次感染后可在体内终生存在。EBV持续存在的主要部位是记忆性B淋巴细胞，但该病毒最初是感染幼稚的B细胞还是记忆性B细胞仍存在争议。我们分析了9例X连锁高免疫球蛋白M(hyper-IgM)综合征患者的EBV感染情况，这些患者由于CD40配体基因突变而没有典型的、类别切换的记忆B细胞群(IGD(-)CD27(+))。我们在67%的病例中发现了EBV感染的证据，这与英国普通人群的感染率(相关年龄段的感染率为60%至70%)相似。我们检测了外周血B细胞中的EBV DNA，其中一例显示感染局限于一小部分非经典的生发中心非依赖性记忆B细胞(IGD(+)CD27(+))。外周血中EBV小RNA、潜伏膜蛋白2和EBV核抗原3C表达的检测表明，该人群中存在完全潜伏的病毒基因表达。系列样本中的EBV DNA分析显示随着时间的推移而变化，表明感染和丢失的周期。我们的结果表明，在没有生发中心反应和经典记忆B细胞群的情况下，可以发生短期的EBV持续。
Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus that persists in the body for life after primary infection. The primary site of EBV persistence is the memory B lymphocyte, but whether the virus initially infects naive or memory B cells is still disputed. We have analyzed EBV infection in nine cases of X-linked hyper-immunoglobulin M (hyper-IgM) syndrome who, due to a mutation in CD40 ligand gene, do not have a classical, class-switched memory B-cell population (IgD(-) CD27(+)). We found evidence of EBV infection in 67% of cases, which is similar to the infection rate found in the general United Kingdom population (60 to 70% for the relevant age range). We detected EBV DNA in peripheral blood B cells and showed in one case that the infection was restricted to the small population of nonclassical, germinal center-independent memory B cells (IgD(+) CD27(+)). Detection of EBV small RNAs, latent membrane protein 2, and EBV nuclear antigen 3C expression in peripheral blood suggests full latent viral gene expression in this population. Analysis of EBV DNA in serial samples showed variability over time, suggesting cycles of infection and loss. Our results demonstrate that short-term EBV persistence can occur in the absence of a germinal center reaction and a classical memory B-cell population.