Ectopic overexpression of maize heat shock transcription factor gene ZmHsf04 confers increased thermo and salt-stress tolerance in transgenic Arabidopsis
Ectopic overexpression of maize heat shock transcription factor gene ZmHsf04 confers increased thermo and salt-stress tolerance in transgenic Arabidopsis
复制标题
玉米热休克转录因子基因 ZmHsf04 的异位过度表达赋予转基因拟南芥增加的热和盐胁迫耐受性
DOI：
10.1007/s11738-017-2587-2
复制
发表时间：
2018-01-01
影响因子：
2.6
通讯作者：
Wu, Jiandong
中科院分区：
文献类型：
--
作者：
Jiang, Yingli;Zheng, Qianqian;Wu, Jiandong
在我们之前的研究中，在玉米中鉴定并分析了25个Hsf基因同源物。然而，这些基因家族成员的确切功能目前尚不清楚。本文报道了玉米ZmHsf 04中一个强热诱导的HsfA 2基因的分离和鉴定，并展示了其在热/盐胁迫反应中的功能。亚细胞定位分析表明ZmHsf 04蛋白定位于细胞核。转录激活数据表明AHA 2结构域蛋白对于ZmHsf 04的转录活性是必需的。此外，ZmHsf 04的功能在拟南芥Col-0转基因植物中进行了详细表征。ZmHsf 04基因的过表达能显著增强热耐受性，并能正向调节短期获得性热耐受。此外，ZmHsf 04显著提高了拟南芥的耐盐性和对脱落酸的敏感性。与野生型相比，ZmHsf 04在Col-0植株中的过表达显著上调了热特异性HSP基因（AtHsp25.3-P、AtHsp18.2-CI和AtHsp 70 B）和胁迫相关基因（AtAPX 2和AtGolS 1）的表达。总之，我们已经表征了转录调节子ZmHsf 04，其在增强植物耐热性和耐盐性中具有重要功能。
In our previous study, 25Hsfgene homologues were identified and analyzed in maize. However, the exact functions of these gene family members are unclear at present. Here, we report the isolation and characterization of a strongly heat-inducedHsfA2gene from maize,ZmHsf04, and show its function in the heat/salt-stress response. Subcellular location assays showed that the ZmHsf04 protein is localized to the nucleus. Transcription activation data demonstrate that an AHA2 domain protein is necessary for the transcriptional activity ofZmHsf04. Furthermore, the function of ZmHsf04 was characterized in detail in transgenic plants ofArabidopsis thalianaCol-0. Overexpression ofZmHsf04inArabidopsisenhanced thermotolerance and positively regulated short-term-acquired thermotolerance. In addition, ZmHsf04 significantly improved salt tolerance and increased the sensitivity to abscisic acid in transgenicArabidopsis. Overexpression ofZmHsf04in Col-0 plants significantly up-regulated the expression of heat-specific HSP genes (AtHsp25.3-P, AtHsp18.2-CI,andAtHsp70B) and stress-related genes (AtAPX2 and AtGolS1) compared to the wild type. In summary, we have characterized a transcriptional regulator, ZmHsf04, which has important functions in enhancing heat and salt tolerance in plants.