Simultaneous detection of two cell populations by two-colour fluorescence and application to the recognition of B-cell determinants
Simultaneous detection of two cell populations by two-colour fluorescence and application to the recognition of B-cell determinants
复制标题
双色荧光同时检测两个细胞群并应用于 B 细胞决定簇的识别
作者：
J. J. Rood;A. Leeuwen;J. Ploem
细胞分离技术，通过免疫荧光和功能测试识别不同标记物的可能性，已经将曾经被认为是同质群体的细胞细分为不同的亚类。这种发展在所谓的单核细胞中最为突出。分类这些亚类（例如，单个核细胞）的固有困难是，通常几乎不可能精确地确定属于某个亚类的细胞的百分比，以及亚类的总和是否等于，低于甚至高于所分析细胞总数的100%。最近一个典型的例子是识别“null”或K单元格1,2。如果我们同时分析用两种或两种以上不同的荧光染料染色的细胞悬浮液，这种分析将大大便利。这是可行的。
CELL separation techniques, the possibility of recognising different markers by immunofluorescence and functional tests, have subdivided cells once thought to be a homogeneous group into different subclasses. This development has been most prominent for the so-called mononuclear cells. A difficulty inherent in the classification of such subclasses (of, for example, mononuclear cells) is that it is often almost impossible to determine precisely what percentage of the cells belongs to a certain subclass, and whether the sum of the subclasses is equal to, lower than or even higher than 100% of the total of the cells analysed. A typical recent example was the identification of the “null” or K cell1,2. Such analysis is greatly facilitated if we analyse a cell suspension stained with two or more different fluorescent dyes visible at the same time. This is feasible.