A GENE ACTIVATED IN MOUSE 3T3-CELLS BY SERUM GROWTH-FACTORS ENCODES A PROTEIN WITH ZINC FINGER SEQUENCES
A GENE ACTIVATED IN MOUSE 3T3-CELLS BY SERUM GROWTH-FACTORS ENCODES A PROTEIN WITH ZINC FINGER SEQUENCES
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DOI：
10.1073/pnas.85.21.7857
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发表时间：
1988-11-01
影响因子：
11.1
通讯作者：
NATHANS, D
中科院分区：
文献类型：
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作者：
CHRISTY, BA;LAU, LF;NATHANS, D
我们最近通过cDNA克隆鉴定了一组在小鼠3T3细胞中被血清或纯化的生长因子快速激活的基因。在这里，我们报道了从这些直接早期基因之一（zif/268）衍生的cDNA（克隆268）编码一个具有三个串联“锌指”序列的蛋白质，这是一类真核转录因子的典型序列。zif/268的mRNA存在于小鼠的许多器官和组织中，尤其是在脑和胸腺组织中含量丰富。zif/268的5”基因组侧翼序列与已知调节蛋白的结合位点相关，包括4个类似于C-fos和actin基因上游血清反应元件（SREs）核心的序列。sre样序列可能是3T3细胞血清刺激后zif/268和fos协同活化的原因。
We have recently identified by cDNA cloning a set of genes that are rapidly activated in mouse 3T3 cells by serum or purified growth factors. Here we report that the cDNA (clone 268) derived from one of these immediate early genes (zif/268) encodes a protein with three tandem "zinc finger" sequences typical of a class of eukaryotic transcription factors. The mRNA of zif/268 is present in many organs and tissues of the mouse and is especially abundant in the brain and thymus tissue. The 5'' genome flanking sequence of zif/268 has sequences related to binding sites for known regulatory proteins, including four sequences that resemble the core of the serum response elements (SREs) upstream of the C-fos and actin genes. The SRE-like sequences could be responsible for the coordinate activation of zif/268 and fos after serum stimulation of 3T3 cells.