Dipeptidase-C in Drosophila melanogaster: genetic, ontogenetic, and tissue-specific variation.
Dipeptidase-C in Drosophila melanogaster: genetic, ontogenetic, and tissue-specific variation.
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果蝇中的二肽酶-C：遗传、个体发生和组织特异性变异。
DOI：
10.1007/bf02399810
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发表时间：
1992
影响因子：
2.4
通讯作者：
Mathes,KD
中科院分区：
文献类型：
--
作者：
Hiraizumi,K;Hourani,CL;Zambarano,MC;Freeman4th,JE;Mathes,KD
Dip-A、Dip-B和Dip-C构成果蝇中三种肽酶的结构基因，与亮氨酸氨肽酶不同。它们的个体发育和组织分布的活动表明，这些酶参与一般的代谢作用，如调节氨基酸和寡肽池，使氨基酸可用于蛋白质合成。对DIP-C活性的染色体置换等基因系的筛选表明，与DIP-A和DIP-B一样，DIP-C存在非连锁活性调节剂。发育概况的二肽酶活动是非常相似的，除了在蛹期，在此期间，DIP-C活性显着低于其他两种酶。二肽酶活性的相互关系各不相同的个体发育，这是一致的，需要协调这些酶在某些发育阶段的表达。幼虫和成虫中二肽酶的组织特异性表达也是相似的，尽管在某些器官和身体部位中DIP-A活性的相对水平不同于DIP-B和DIP-C。染色体代换系之间的二肽酶活性的一些差异似乎是系统的，而另一些则是发育阶段特异性和组织特异性的。DIP-C活性的第二和第三染色体变体在其组织分布中不同。这与其他果蝇酶在自然种群中存在时间和空间变异是一致的。
Dip-A, Dip-B, andDip-Cconstitute structural genes for three peptidic enzymes inDrosophila melanogasterdistinct from the leucine aminopeptidases. Their ontogenetic and tissue distributions of activities suggest the involvement of these enzymes in a general metabolic role, such as the regulation of amino acid and oligopeptide pools to make amino acids available for protein synthesis. Screening of chromosome substitution isogenic lines for DIP-C activity indicated that, like DIP-A and DIP-B, unlinked activity modifiers exist forDip-C. The developmental profiles of dipeptidase activities are very similar, except in the pupal stage, during which DIP-C activity is markedly low compared to the other two enzymes. Intercorrelations of dipeptidase activities vary ontogenetically, which is consistent with the need for coordinate expression of these enzymes during certain developmental stages. Tissue-specific expressions of dipeptidases in larvae and adults are also similar, although the relative levels of DIP-A activity differ from those of DIP-B and DIP-C in certain organs and body parts. Some of the differences among chromosome substitution lines for dipeptidase activities appear to be systemic, while others are developmental stage-specific and tissue-specific. Second- and third-chromosome variants for DIP-C activity differed in their tissue distribution. This is consistent with the presence of temporal and spatial variants in natural populations for otherDrosophilaenzymes.