An experimental study on collagen content and biomechanical properties of sclera after posterior sclera reinforcement
An experimental study on collagen content and biomechanical properties of sclera after posterior sclera reinforcement
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DOI：
10.1016/j.clinbiomech.2007.10.013
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发表时间：
2008-01-01
影响因子：
1.8
通讯作者：
Zhang Quanyou
中科院分区：
文献类型：
--
作者：
Chen Weiyi;Wang Xiaojun;Zhang Quanyou
背景在人类和动物模型中，病理性近视的发展都与巩膜胶原堆积减少、巩膜变薄和巩膜组织丢失有关。后巩膜加固术被认为是治疗病理性近视的有效方法。然而，胶原油在PSR手术中的可能作用及巩膜强化机制尚不清楚。对正常成年新西兰大白兔的眼行PSR手术。采用人巩膜作为增强材料。术后1、2、3、6、9个月用酶解法和组织学方法检测巩膜羟脯氨酸(Hyp)的合成和胶原纤维的排列。使用Instron试验机研究了巩膜的弹性模量。结果表明，术后1个月，加强型巩膜的弹性模量值和Hyp含量均较低，随后逐渐恢复到生理水平。这两项指标在9个月时接近正常对照组。这些结果表明，巩膜弹性模量与术后巩膜Hyp含量和胶原纤维排列的变化有关。从生物学和生物力学两个方面证实了PSR手术的疗效。(C)2007爱思唯尔有限公司。保留所有权利。
Background The development of pathological myopia is associated with reduced scleral collagen accumulation, scleral thinning, and loss of scleral tissue, in both humans and animal models. Posterior scleral reinforcement (PSR) was considered as an effective way for treating pathological myopia. Yet it is not well understood the possible role of collagen oil the sclera reinforcement mechanisms in the PSR surgery.Methods. PSR surgery was performed on the normal adult New Zealand white rabbits eyes. Human sclera was used as reinforcement materials. At 1, 2, 3, 6, 9 months after the PSR surgery, scleral hydroxyproline (Hyp) synthesis and collagen fibers arrangement were determined by enzymolysic hydrolysis assay and histological morphology technique. An Instron test machine was used to investigate the elastic modulus of sclera.Findings. It was found that the elastic modulus and Hyp content of reinforced sclera were lower at first month after surgery, and then gradually up to physiological level in the following months. Those two indexes were close to that of the normal control groups at 9 months.Interpretation. These findings indicate that sclera elastic modulus was associated with both change of Hyp content and collagen fibers arrangement after PSR. The therapeutic effect of PSR surgery was confirmed not only from biological but also biomechanical aspects. (C) 2007 Elsevier Ltd. All rights reserved.