Increased Cerebrospinal Fluid Amyloid-β During Sleep Deprivation in Healthy Middle-Aged Adults Is Not Due to Stress or Circadian Disruption
Increased Cerebrospinal Fluid Amyloid-β During Sleep Deprivation in Healthy Middle-Aged Adults Is Not Due to Stress or Circadian Disruption
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DOI：
10.3233/jad-191122
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发表时间：
2020-01-01
影响因子：
4
通讯作者：
Lucey, Brendan P.
中科院分区：
文献类型：
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作者：
Blattner, Margaret S.;Panigrahi, Sunil K.;Lucey, Brendan P.
背景：小鼠间质液和人类脑脊液 (CSF) 中可溶性淀粉样蛋白 - β (Aβ) 的浓度随着睡眠-觉醒周期而振荡。此外，睡眠剥夺期间脑脊液中 Aβ 的浓度会增加。压力和生物钟的破坏被认为是增加 CSF A β 水平的额外机制。皮质醇是压力的标志物，具有内源性昼夜节律。葡萄糖和乳酸等其他因素与睡眠-觉醒活动和/或 Aβ 的变化有关。 目的：在这项探索性研究中，我们使用之前研究中收集的样本来检查睡眠剥夺如何影响健康中年成年人 (N = 11) 血浆和脑脊液中的 Aβ、皮质醇、乳酸和葡萄糖。 方法：11 名没有睡眠障碍证据的认知正常的参与者被纳入研究中。 随机分为睡眠剥夺组或正常睡眠控制组。所有参与者都被邀请重复这项研究。在 36 小时内，每隔 2 小时测量血浆和脑脊液中的皮质醇、乳酸、葡萄糖和 Aβ。在计算中观、振幅、末期和其他参数之前，将所有浓度标准化为平均值。结果：一晚睡眠剥夺使脑脊液中 Aβ 的夜间浓度增加约 10%，但在睡眠剥夺和对照条件之间，不会显着影响血浆或脑脊液中的皮质醇、乳酸或葡萄糖浓度。结论：这些数据表明，睡眠剥夺与睡眠剥夺相关 CSF A β 的变化不是由皮质醇测量的压力或昼夜节律紊乱介导的。
Background: Concentrations of soluble amyloid-beta (A beta) oscillate with the sleep-wake cycle in the interstitial fluid of mice and cerebrospinal fluid (CSF) of humans. Further, the concentration of A beta in CSF increases during sleep deprivation. Stress and disruption of the circadian clock are additional mechanisms hypothesized to increase CSF A beta levels. Cortisol is a marker for stress and has an endogenous circadian rhythm. Other factors such as glucose and lactate have been associated with changes in sleep-wake activity and/or A beta.Objective: In this exploratory study, we used samples collected in a previous study to examine how sleep deprivation affects A beta, cortisol, lactate, and glucose in plasma and CSF from healthy middle-aged adults (N = 11).Methods: Eleven cognitively normal participants without evidence of sleep disturbance were randomized to sleep deprivation or normal sleep control. All participants were invited to repeat the study. Cortisol, lactate, glucose, and A beta were measured in 2-h intervals over a 36-h period in both plasma and CSF. All concentrations were normalized to the mean prior to calculating mesor, amplitude, acrophase, and other parameters.Results: One night of sleep deprivation increases the overnight concentration of A beta in CSF approximately 10%, but does not significantly affect cortisol, lactate, or glucose concentrations in plasma or CSF between the sleep-deprived and control conditions.Conclusion: These data suggest that sleep deprivation-related changes in CSF A beta are not mediated by stress or circadian disruption as measured by cortisol.