Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology

Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology
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DOI:
10.1023/a:1000669317571
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发表时间:
1998-01-01
影响因子:
2.6
通讯作者:
Smalla, K
Smalla, K
中科院分区:
生物学3区
文献类型:
--
作者:
Muyzer, G;Smalla, K

文献摘要

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本文介绍了变性梯度凝胶电泳法(DGGE)和温度梯度凝胶电泳法(TGGE)在微生物生态学中的应用现状,并讨论了这些技术的潜力和局限性,指出了它们在生态学研究中的重要性。
Here, the state of the art of the application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be presented, Furthermore, the potentials and limitations of these techniques will be discussed, and it will be indicated why their use in ecological studies has become so important.