IL8 and IL16 levels indicate serum and plasma quality
IL8 and IL16 levels indicate serum and plasma quality
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DOI：
10.1515/cclm-2017-1047
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发表时间：
2018-07-01
影响因子：
6.8
通讯作者：
Betsou, Fay
中科院分区：
文献类型：
--
作者：
Kofanova, Olga;Henry, Estelle;Betsou, Fay
背景：较长的预离心时间会改变血清和血浆样品的质量。已经确定了样品处理中此类延迟的标记，从而影响了样品质量。方法：筛选血清、EDTA 血浆和柠檬酸盐血浆样品中的 20 种细胞因子，以了解室温下延长血液预离心延迟引起的浓度变化。变化最大的两种细胞因子进一步验证了其在识别延长预离心时间的血清或血浆样本方面的“诊断性能”。 结果：在本研究中，使用 R&D Systems ELISA 试剂盒，EDTA 血浆样本和预离心延迟时间超过 24 h 的血清样本的 IL16 浓度分别高于 313 pg/mL，IL8 浓度分别高于 125 pg/mL。预离心延迟超过 48 小时的 EDTA 血浆样品的 IL16 浓度高于 897 pg/mL，柠檬酸盐血浆样品的 IL8 浓度高于 21.5 pg/mL，血清样品的 IL8 浓度高于 528 pg/mL。结论：这些基于简单和市售 ELISA 测定的强大而准确的工具可以极大地促进未记录的血清和血浆遗留收集品的鉴定。预分析。
Background: Longer pre-centrifugation times alter the quality of serum and plasma samples. Markers for such delays in sample processing and hence for the sample quality, have been identified.Methods: Twenty cytokines in serum, EDTA plasma and citrate plasma samples were screened for changes in concentration induced by extended blood pre-centrifugation delays at room temperature. The two cytokines that showed the largest changes were further validated for their " diagnostic performance" in identifying serum or plasma samples with extended pre-centrifugation times.Results: In this study, using R& D Systems ELISA kits, EDTA plasma samples and serum samples with a pre-centrifugation delay longer than 24 h had an IL16 concentration higher than 313 pg/mL, and an IL8 concentration higher than 125 pg/mL, respectively. EDTA plasma samples with a pre-centrifugation delay longer than 48 h had an IL16 concentration higher than 897 pg/mL, citrate plasma samples had an IL8 concentration higher than 21.5 pg/mL and serum samples had an IL8 concentration higher than 528 pg/mL.Conclusions: These robust and accurate tools, based on simple and commercially available ELISA assays can greatly facilitate qualification of serum and plasma legacy collections with undocumented pre-analytics.