Neurogenesis in the ependymal layer of the adult rat 3rd ventricle
Neurogenesis in the ependymal layer of the adult rat 3rd ventricle
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DOI：
10.1016/j.expneurol.2004.12.021
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发表时间：
2005-04-01
影响因子：
5.3
通讯作者：
Ide, C
中科院分区：
文献类型：
--
作者：
Xu, Y;Tamamaki, N;Ide, C
已在成年哺乳动物大脑的有限区域中描述了神经发生。在这项研究中，我们发现第三脑室的室管膜层是成年大鼠大脑中的神经源性区域。第三脑室的 Dil 标记表明神经祖细胞源自成人第三脑室室管膜层的细胞。 bFGF 给药促进了室管膜层这些祖细胞的有丝分裂。 BrdU 给药、巢蛋白/GFAP 免疫组织化学和 GFP 重组腺病毒感染 (vGFP) 标记的组合表明，至少一些单细胞可能是第三脑室室管膜层中的神经祖细胞。 vGFP追踪表明，神经祖细胞可能已经从第三脑室迁移到下丘脑实质，在那里它们通过形成突触整合到神经网络中。此外，一些BrdU(+)神经元对下丘脑中的食欲素A具有免疫反应性。这些结果表明，神经祖细胞存在于成年大鼠第三脑室的室管膜层中，并且它们可能分化为在下丘脑中发挥功能的神经元。 (c) 2004 Elsevier Inc. 保留所有权利。
Neurogenesis has been described in limited regions of the adult mammalian brain. In this study, we showed that the ependymal layer of the 3rd ventricle is a neurogenic region in the adult rat brain. Dil labeling of the 3rd ventricle revealed that neural progenitor cells were derived from cells at the ependymal layer of the adult 3rd ventricle. The mitosis of these progenitor cells at the ependymal layer was promoted by bFGF administration. Combination of BrdU administration, nestin/GFAP immunohistochemistry, and labeling by GFP-recombinant adenoviral infection (vGFP) indicated that at least some tanycytes might be neural progenitor cells in the ependymal layer of the 3rd ventricle. Tracing by vGFP indicated that neural progenitor cells may have migrated from the 3rd ventricle to the hypothalamic parenchyma, where they were integrated into neural networks by forming synapses. In addition, some BrdU(+) neurons had immunoreactivity for orexin A in the hypothalamus. These results indicate that neural progenitor cells exist in the ependymal layer of the adult rat 3rd ventricle and that they may differentiate into neurons functioning in the hypothalamus. (c) 2004 Elsevier Inc. All rights reserved.