Reductions in laminin beta2 mRNA translation are responsible for impaired IGFBP-5-mediated mesangial cell migration in the presence of high glucose.
Reductions in laminin beta2 mRNA translation are responsible for impaired IGFBP-5-mediated mesangial cell migration in the presence of high glucose.
复制标题
在高葡萄糖存在下，层粘连蛋白β2 mRNA翻译的减少导致IGFBP-5介导的系膜细胞迁移受损。
DOI：
10.1152/ajprenal.00483.2009
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Abrass,ChristineK
中科院分区：
文献类型：
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作者：
Schaeffer,Valerie;Hansen,KimM;Morris,DavidR;Abrass,ChristineK
Insulin-like growth factor binding protein-5 (IGFBP-5) mediates mesangial cell migration through activation of cdc42, and laminin421 binding to α6β1-integrin (Berfield AK, Hansen KM, Abrass CK.Am J Physiol Cell Physiol291: C589–C599, 2006). Because glomerular expression of laminin β2is reduced in diabetic rats (Abrass CK, Spicer D, Berfield AK, St. John PL, Abrahamson DR.Am J Pathol151: 1131–1140, 1997), we directly examined the effect of hyperglycemia on mesangial cell migration and laminin β2 expression. Migration mediated by IGFBP-5 is impaired in the presence of 25 mM glucose. This reduction in migration was found to result from a loss in mesangial cell synthesis of laminin421, and IGFBP-5-induced migration could be restored by replacing laminin421. Additional studies showed that there was selective reduction in mRNA translation of laminin β2 in the presence of high glucose. Preserved synthesis of laminin β1 indicates that not all proteins are reduced by high glucose and confirms prior data showing that laminin411 cannot substitute for laminin421 in IGFBP-5-mediated migration. Given the importance of mesangial migration in the reparative response to diabetes-associated mesangiolysis, these findings provide new insights into abnormalities associated with diabetic nephropathy and the potential importance of differential control of protein translation in determination of alterations of protein expression.
