Differential expression of SAPK isoforms in the rat brain. An in situ hybridisation study in the adult rat brain and during post-natal development
Differential expression of SAPK isoforms in the rat brain. An in situ hybridisation study in the adult rat brain and during post-natal development
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DOI：
10.1016/s0169-328x(98)00166-1
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发表时间：
1998-09-18
期刊：
影响因子：
--
通讯作者：
Ferraguti, F
中科院分区：
文献类型：
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作者：
Carboni, L;Carletti, R;Ferraguti, F
MAPK通路将多种细胞外信号转化为细胞反应。尽管它们具有多效性和无处不在的分布，但令人惊讶的是，人们对它们在神经细胞通讯网络中的作用知之甚少。作为阐明MAPK通路在神经元信号传导中的作用的第一步，我们研究了SAPK/JNK的三种亚型SAPK α /JNK2、SAPK β /JNK3和SAPK γ /JNK1的分布。SAPK/JNK是应激激活的MAPK亚家族。我们使用原位杂交技术比较了成年和发育中的大鼠脑中三种主要亚型的mRNA定位。在成人大脑中，SAPK α和β广泛但不均匀地分布，再现了不区分同种异构体的探针模式。不同的是，SAPK γ探针的高标记仅局限于虹膜内核和内侧缰。海马呈中度染色。在产后发育过程中，SAPK β表现出与成人相同的定位。然而，光密度的半定量分析显示mRNA水平有显著差异。成年大鼠的SAPK γ信号较弱，而新生大鼠的标记信号强烈且分布广泛。在发育过程中，SAPK γ mRNA水平下降，达到成人中检测到的低信号。这些结果表明，在中枢神经系统中，sapk型MAP激酶具有重要的生理功能，特别是在产后发育过程中。成年大鼠脑中SAPK同种异构体的不同分布模式支持了三种SAPK基因产物执行不同功能的假设。(C) 1998 Elsevier Science B.V.版权所有
MAPK pathways transduce a broad variety of extracellular signals into cellular responses. Despite their pleiotropic effects and their ubiquitous distribution, surprisingly little is known about their involvement in the communication network of nerve cells. As a first step to elucidate the role of MAPK pathways in neuronal signalling, we studied the distribution of SAPK alpha/JNK2, SAPK beta/JNK3, and SAPK gamma/JNK1, three isoforms of SAPK/JNK, a stress-activated MAPK subfamily. We compared the mRNA localisation of the three main isoforms in the adult and developing rat brain using in situ hybridisation. In the adult brain, SAPK alpha and beta were widely but heterogeneously distributed, reproducing the pattern of a probe that does not discriminate the isoforms. Differently, high labelling for the SAPK gamma probe was exclusively localised in the endopiriform nucleus and medial habenula. Intermediate staining was detected in the hippocampus. During post-natal development, SAPK beta showed the same localisation as in the adult. Nevertheless, the semi-quantitative analysis of optical densities showed significantly different mRNA levels. In the adult, SAPK gamma signal was weak, whereas in newborn rats the labelling was intense and widely distributed. SAPK gamma mRNA levels decreased during development, to reach the low signals detected in the adult. These results suggest that in the central nervous system SAPK-type MAP kinases perform significant physiological functions which are particularly relevant during post-natal development. The distinct distribution patterns of SAPK isoforms in the adult rat brain support the hypothesis that separate functions are performed by the products of the three SAPK genes. (C) 1998 Elsevier Science B.V. All rights reserved.