The MADS-domain protein, PPM2 preferentially occurs in gametangia and sporophytes of the moss Physcomitrella patens
The MADS-domain protein, PPM2 preferentially occurs in gametangia and sporophytes of the moss Physcomitrella patens
复制标题
DOI：
10.1016/j.gene.2007.05.016
复制
发表时间：
2007-10-01
期刊：
影响因子：
3.5
通讯作者：
Muenster, Thomas
中科院分区：
文献类型：
--
作者：
Faigl, Vanessa Quodt Wolfram;Saedler, Heinz;Muenster, Thomas
迄今为止，尽管已经从多种物种中分离和鉴定了许多基因，但非种子植物中 MADS 结构域转录因子的功能仍然很大程度上难以捉摸。在我们的研究中，我们利用立碗藓在高效同源重组方面提供的独特技术特性，分析了来自苔藓立碗藓的经典 MIKC 型 MADS-box 基因 PPM2。我们确定了 mRNA 和蛋白质分布，并对基因组位点进行了靶向破坏，以进行 PPM2 的功能分析。尽管普遍存在的表达较弱，但 PPM2 蛋白主要存在于雄性和雌性 gamtangia 以及发育中的孢子体的基部部分中。因此，PPM2 似乎在苔藓生命周期的单倍体和二倍体阶段都发挥作用。这种情况反映了基因募集的进化过渡状态，从祖先的配子体世代到衍生的孢子体世代，后者在气管植物中占主导地位。然而，PPM2 基因的敲除并没有引起各自结构的明显表型变化。讨论了我们的发现对于理解植物中 MADS-box 转录因子进化史的影响。 (c) 2007 Elsevier B.V. 保留所有权利。
To date, the function of MADS-domain transcription factors in non-seed plants remains largely elusive, although a number of genes have been isolated and characterized from a variety of species. In our study we analyzed PPM2, a classical MIKC-type MADS-box gene from the moss Physcomitrella patens, taking advantage of the unique technical properties Physcomitrella offers in terms of efficient homologous recombination. We determined mRNA and protein distribution and performed targeted disruption of the genomic locus for functional analysis of PPM2. Despite weak ubiquitous expression, PPM2 protein is mostly found in male and female gamtangia and basal parts of developing sporophytes. Therefore, PPM2 seems to function in both the haploid and the diploid phase of the moss life cycle. This situation reflects an evolutionary transition state of gene recruitment from an ancestral gamtophytic generation into a derived sporophytic generation which became dominating in tracheophytes. However, a knock-out of the PPM2 gene did not cause visible phenotypical changes in the respective structures. The implications of our findings for the understanding of the evolutionary history of MADS-box transcription factors in plants are discussed. (c) 2007 Elsevier B.V. All rights reserved.