Cell proliferation in the process of oviducal tissue remodeling during induced molting in hens.
Cell proliferation in the process of oviducal tissue remodeling during induced molting in hens.
复制标题
母鸡诱导换羽过程中输卵管组织重塑过程中的细胞增殖。
作者：
B. Heryanto;Y. Yoshimura;T. Tamura
观察了诱导蜕皮过程中输卵管组织重塑和钙结合蛋白d28k （CaBP-D28K）的动态变化。分别对脱羽前、停产后7、10、13、16 d和脱羽后的蛋鸡进行了卵管组织检测。采用增殖细胞核抗原（PCNA）免疫细胞化学和Western blot检测CaBP-D28K的增殖频率和免疫反应性。在蜕皮前和停止产卵后7 d，大鼠、峡部和壳腺粘膜上皮中pcna阳性细胞的相对频率较低。在戒烟后10 d和13 d，大颈部和峡部明显增加，随后在戒烟后16 d略有下降。停止后10、13、16 d，壳腺内的频率保持较高。恢复产蛋后，各节段出现pcna阳性细胞的频率降低。在管状腺所在的大鼠和峡部间质中，停止产卵10 d后和停止产卵7 d后，pcna阳性细胞的频率显著高于脱毛前和脱毛后。在蜕皮后和停止蜕皮16 d的母鸡中，蜕皮频率下降到与蜕皮前母鸡相同的水平。在壳腺中，pna阳性细胞的频率在第10、13和16天较高，在蜕皮后降低。在蜕皮前、蜕皮后和雏鸡的壳腺中均观察到CaBP-D28K的单一免疫沉淀带，且蜕皮后和雏鸡的条带密度大于蜕皮前的条带。我们认为，输卵管组织的重建是通过将旧的腺细胞替换为来自粘膜上皮和未折叠腺细胞的新细胞来实现的。这种壳腺组织的年轻化可能导致CaBP-D28K诱导的改善。
Tissue remodeling and calcium binding protein-D28K (CaBP-D28K) dynamics were examined in the oviduct relative to induced molting. The oviducal tissues of premolting, 7, 10, 13, and 16 d after cessation of laying, and postmolting hens were examined. Frequency of proliferating cells and immunoreactive CaBP-D28K molecules were identified by immunocytochemistry for proliferating cell nuclear antigen (PCNA) and Western blot for CaBP-D28K. The relative frequency of PCNA-positive cells in the mucosal epithelium of the magnum, isthmus, and shell gland was low in premolting and 7 d after the cessation of egg laying. In the magnum and isthmus it was markedly increased in 10 and 13 d after cessation, followed by a slight decrease at 16 d after cessation. The frequency in the shell gland was kept high 10, 13, and 16 d after cessation. The frequency of PCNA-positive cells in each segment was decreased when the birds resumed laying. In the stroma of magnum and isthmus where the tubular glands were located, the frequency of PCNA-positive cells was significantly increased 10 d after the cessation of egg laying relative to premolting and 7 d after cessation. In postmolting hens and in hens 16 d after cessation, the frequency was decreased to a same level to that of premolting hen. In the shell gland, the frequency of PCNA-positive cells was high at 10, 13, and 16 d, and diminished in postmolting hen. Single immunoprecipitate band for CaBP-D28K was observed in the shell gland of premolting, postmolting, and younger hens, whereas the density of bands was greater in postmolting hens and younger hens than in premolting hens. We suggest that the oviducal tissues are remodeled by replacing the old glandular cells with new ones that are derived from the mucosal epithelium and uninvoluted glandular cells. Such rejuvenation of shell gland tissue may lead the improvement of CaBP-D28K induction.