Identification of a Rho family specificguanine nucleotide exchange factorFLJ00018, as a novel actin-bindingprotein
Identification of a Rho family specificguanine nucleotide exchange factorFLJ00018, as a novel actin-bindingprotein
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鉴定 Rho 家族特异性鸟嘌呤核苷酸交换因子 FLJ00018，作为一种新型肌动蛋白结合蛋白
DOI：
10.1016/j.cellsig.2012.09.015
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
H.
中科院分区：
文献类型：
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作者：
Sato;K.;Handa;H.;Kimura;M.;Okano,Y.;Nagaoka;H.;Nagase;T.;Sugiyama,T.;Kitade;Y.;and Ueda;H.
FLJ 00018/PLEKHG 2是Rho家族小GTP酶的鸟嘌呤核苷酸交换因子。FLJ 00018直接被异源三聚体G蛋白Gβγ亚基激活。使用双杂交筛选，我们已经确定了非肌肉细胞溶质肌动蛋白作为FLJ 00018的结合伴侣。结果表明，FLJ 00018在N端（150-283个氨基酸）和C端（465-1386个氨基酸）存在两个肌动蛋白结合区。非肌肉细胞溶质肌动蛋白的过表达减弱了FLJ 00018诱导的血清反应元件依赖性基因转录。这些结果表明，非肌肉细胞溶质肌动蛋白可能是FLJ 00018通过其与Dbl同源结构域的相互作用的负调节剂。
FLJ00018/PLEKHG2 is a guanine nucleotide exchange factor for the Rho family small GTPases. FLJ00018 is directly activated by heterotrimeric G protein Gβγ subunits. Using two-hybrid screening, we have identified non-muscle cytosolic actin as a binding partner of FLJ00018. We found that there were two actin-binding regions in FLJ00018 at the N-terminal region (150–283 amino acids) and at the C-terminal region (465–1386 amino acids). The overexpression of non-muscle cytosolic actin attenuated the FLJ00018-induced serum response element-dependent gene transcription. These results suggest that non-muscle cytosolic actin may be a negative regulator of FLJ00018 through its interaction with the Dbl homology domain.