SPECIES-SPECIFIC GRAZING RATES OF HETEROTROPHIC DINOFLAGELLATES IN OCEANIC WATERS, MEASURED WITH A DUAL-LABEL RADIOISOTOPE TECHNIQUE
SPECIES-SPECIFIC GRAZING RATES OF HETEROTROPHIC DINOFLAGELLATES IN OCEANIC WATERS, MEASURED WITH A DUAL-LABEL RADIOISOTOPE TECHNIQUE
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DOI：
10.1007/bf00397808
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发表时间：
1985-01-01
期刊：
影响因子：
2.4
通讯作者：
SWIFT, E
中科院分区：
文献类型：
--
作者：
LESSARD, EJ;SWIFT, E
一个双同位素的方法来测量放牧率和食物偏好的个别物种的异养甲藻从自然种群，收集从斜坡，墨西哥湾流，马尾藻海和从冰岛到新英格兰[美国]，在1983年的一个横断面。同位素方法测量微型浮游动物的摄食率，这些浮游动物不能根据大小在自然种群中分离。氚化胸苷和14 C-碳酸氢盐分别用于标记天然异养和自养食物。本文报道了原多甲藻属（Protoperidinium depressum）、叉甲藻属（P. divergens）、大洋多甲藻属（P. oceanicum）、秀丽多甲藻属（P. elegans）、卵形多甲藻属（P. ovatum）、掌足多甲藻属（Podolampas palmipes）、棘多甲藻属（P. spinifer）、双足多甲藻属（P. bipes）、双足多甲藻属（D. dipes）等9种海洋甲藻。以异养和自养食物颗粒两者喂养的，基于3 H掺入的清除率为0.4-8.0 μ l细胞-1h-1，基于14 C掺入的清除率为0.0 - 28.3 μ l细胞-1h-1。两个甲藻物种，卵形原多甲藻和Podolampas palmipes，只吃3 H标记的食物颗粒。几种甲藻以用3 H-胸苷预标记的细菌（< 1 μ m）为食。异养甲藻和纤毛虫的清除率相似，并在文献报道的tintinnid纤毛虫清除率的范围内。在海洋沃茨中，异养甲藻可能与浮游植物和细菌的微异养食草动物具有同样重要的影响。
A dual-isotope method was developed to measure grazing rates and food preferences of individual species of heterotrophic dinoflagellates from natural populations, collected from the Slope, Gulf Stream, and Sargasso Sea and from a transect from Iceland to New England [USA], in 1983. The isotope method measures the grazing rates of microzooplankton which cannot be separated in natural populations on the basis of size. Tritiated-thymidine and 14C-bicarbonate were used to label natural heterotrophic and autotrophic food, respectively. Nine oceanic dinoflagellate species in the genera Protoperidinium, Podolampas and Diplopsalis [Protoperidinium depressum, P. divergens, P. oceanicum, P. elegans, P. ovatum, Podolampas palmipes, P. spinifer, P. bipes, D. lenticulata] fed on both heterotrophic and autotrophic food particles with clearance rates of 0.4-8.0 .mu.l cell-1 h-1, based on 3H incorporation, and 0.0 to 28.3 .mu.l cell-1 h-1, based on 14C incorporation. Two dinoflagellate species, Protoperidinium ovatum and Podolampas palmipes, fed only on 3H-labeled food particles. Several species of dinoflagellates fed on bacteria (< 1 .mu.m) which had been prelabeled with 3H-thymidine. The clearance rates of heterotrophic dinoflagellates and ciliates were similar and within the range of tintinnid ciliate clearance rates reported in the literature. Heterotrophic dinoflagellates may have an equally important impact as microheterotrophic grazers of phytoplankton and bacteria in oceanic waters.