A quantitative ultrastructural analysis of satellite cells in denervated fast and slow muscles of the mouse
A quantitative ultrastructural analysis of satellite cells in denervated fast and slow muscles of the mouse
复制标题
小鼠快肌和慢肌失神经卫星细胞的定量超微结构分析
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
M. H. Snow
中科院分区：
文献类型：
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作者：
M. H. Snow
在成年小鼠的趾长伸肌（EDL）和比目鱼肌中，在结扎并去除一段坐骨神经后3 ~ 65天，对位于失神经支配肌纤维外板层和肌膜之间的单核细胞进行定量和超微结构检查。在第一个2周postdenervation，单核细胞在失神经支配的肌肉形态学上难以区分的卫星细胞中观察到的控制肌肉。然而，随着时间的推移，这些卫星样细胞中的许多细胞似乎更加活跃，这可以通过其核质比的降低和其常染色质材料的平均百分比的增加来证明。卫星细胞的数量（表示为卫星细胞核与卫星细胞核加肌核的比率）直到失神经支配后30天才显著增加，此时比目鱼肌的平均百分比从对照值4.1- 8.5%上升，而EDL从1.2- 4.1%上升。小直径，推测再生，肌纤维偶尔观察到，但只有在30天后去神经。超微结构证据加上肌核和卫星细胞核之间常染色质分布的比较支持以下概念：去神经支配期间卫星样细胞数量的增加更可能是由于卫星细胞增殖，而不是利用预先存在的肌核形成单核片段。
Mononucleated cells located between the external lamina and sarcolemma of denervated muscle fibers within the extensor digitorum longus (EDL) and soleus muscles of adult mice were quantified and examined ultrastructurally from 3 to 65 days after ligating and removing a section of the sciatic nerve. During the first 2 weeks postdenervation, mononucleated cells in denervated muscles were morphologically indistinguishable from satellite cells observed in control muscles. With time, however, many of these satellite‐like cells appeared more active as evidenced by a decrease in their nucleocytoplasmic ratio and an increase in their mean percentage of euchromatin material. The number of satellite cells (expressed as a ratio of satellite cell nuclei to satellite cell nuclei plus myonuclei) did not increase significantly until 30 days postdenervation, at which time the mean percentage for the soleus muscle had risen from a control value of 4.1–8.5%, and for the EDL from 1.2–4.1%. Smalldiameter, presumably regenerating, myofibers were occasionally observed but only after 30 days denervation. The ultrastructural evidence plus comparisons of euchromatin distributions between myonuclei and satellite cell nuclei support the concept that an increase in the number of satellite‐like cells during denervation is more likely due to satellite cell proliferation than to the formation of mononucleated fragments utilizing preexisting myonuclei.