Extracytoplasmic Function σ Factors as Tools for Coordinating Stress Responses.
Extracytoplasmic Function σ Factors as Tools for Coordinating Stress Responses.
复制标题
DOI：
10.3390/ijms22083900
复制
发表时间：
2021-04-09
影响因子：
5.6
通讯作者：
Reyes-Ramírez F
中科院分区：
文献类型：
--
作者：
de Dios R;Santero E;Reyes-Ramírez F
细菌核心RNA聚合酶（RNAP）能够与不同的σ因子相互作用，从而形成具有不同特异性的多种全酶，这是协调基因表达重编程的有力工具。细胞质外功能σ因子（Extracytoplasmic function σ factors，ECFs）是σ因子家族中最大、种类最多的一类因子，常参与应激反应。根据系统发育关系和基因组背景将ECFs分为157个不同的组，揭示了它们的多样性。在这里，我们聚集了55个ECF组与实验研究的代表成两大类的压力反应。剩下的102组仍然缺乏任何机械或功能的见解，代表了无数的系统尚未探索。在这项工作中，我们回顾了ECFs的主要特点，并讨论了控制其生产和活动的不同机制，以及它们如何导致功能性应激反应。最后，我们专注于更详细的两个良好的特征ECFs，检测和响应应力的机制是复杂的和完全不同的：大肠杆菌RpoE，这是最好的特征ECF和其结构和功能的研究提供了关键的见解ECF-RNAP全酶的转录起始，和ECF 15型EcfG，在Alphaproteobacteria的一般应力响应的主调节器。
The ability of bacterial core RNA polymerase (RNAP) to interact with different σ factors, thereby forming a variety of holoenzymes with different specificities, represents a powerful tool to coordinately reprogram gene expression. Extracytoplasmic function σ factors (ECFs), which are the largest and most diverse family of alternative σ factors, frequently participate in stress responses. The classification of ECFs in 157 different groups according to their phylogenetic relationships and genomic context has revealed their diversity. Here, we have clustered 55 ECF groups with experimentally studied representatives into two broad classes of stress responses. The remaining 102 groups still lack any mechanistic or functional insight, representing a myriad of systems yet to explore. In this work, we review the main features of ECFs and discuss the different mechanisms controlling their production and activity, and how they lead to a functional stress response. Finally, we focus in more detail on two well-characterized ECFs, for which the mechanisms to detect and respond to stress are complex and completely different: Escherichia coli RpoE, which is the best characterized ECF and whose structural and functional studies have provided key insights into the transcription initiation by ECF-RNAP holoenzymes, and the ECF15-type EcfG, the master regulator of the general stress response in Alphaproteobacteria.
登录
查看更多内容
影响因子：
14.9
作者：
Casas-Pastor D;Müller RR;Jaenicke S;Brinkrolf K;Becker A;Buttner MJ;Gross CA;Mascher T;Goesmann A;Fritz G
通讯作者：
Fritz G
影响因子：
16
作者：
Campbell, Elizabeth A.;Greenwell, Roger;Darst, Seth A.
通讯作者：
Darst, Seth A.
影响因子：
3.7
作者：
Britos L;Abeliuk E;Taverner T;Lipton M;McAdams H;Shapiro L
通讯作者：
Shapiro L
影响因子：
--
作者：
Barchinger, Sarah E;Ades, Sarah E
通讯作者：
Ades, Sarah E
影响因子：
16
作者：
Campbell, EA;Tupy, JL;Darst, SA
通讯作者：
Darst, SA