Activity in mice of recombinant BCG-EgG1Y162 vaccine for Echinococcus granulosus infection.
Activity in mice of recombinant BCG-EgG1Y162 vaccine for Echinococcus granulosus infection.
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重组 BCG-EgG1Y162 疫苗对细粒棘球绦虫感染的小鼠活性
DOI：
10.1080/21645515.2015.1064564
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发表时间：
2016
影响因子：
4.8
通讯作者：
Ding J
中科院分区：
文献类型：
--
作者：
Ma X;Zhao H;Zhang F;Zhu Y;Peng S;Ma H;Cao C;Xin Y;Yimiti D;Wen H;Ding J
囊型包虫病是由细粒棘球蚴引起的一种人畜共患寄生虫病，在世界范围内均有分布。该病很难治疗，手术切除是唯一根治的治疗方法。在高流行地区，人类接种疫苗被认为是保护社区免受疾病侵害的一种方式。本研究以rBCG-EgG 1 Y162免疫BALB/c小鼠，通过ELISA方法检测重组蛋白特异性IgG和IgE的水平，结果表明rBCG-EgG 1 Y162诱导了较强的特异性细胞免疫和体液免疫应答。体外研究表明，rBCG-EgG 1 Y162疫苗不仅能促进脾细胞增殖，而且能激活T细胞。此外，rBCG-EgG 1 Y162诱导小鼠对细粒棘球蚴继发感染的保护作用。
Cystic hydatid disease is a zoonotic parasitic disease caused by Echinococcus granulosus which is distributed worldwide. The disease is difficult to treat with surgery removal is the only cure treatment. In the high endemic areas, vaccination of humans is believed a way to protect communities from the disease. In this study we vaccinated BALB/c mice with rBCG-EgG1Y162, and then detected the level of IgG and IgE specifically against the recombinant protein by ELISA, rBCG-EgG1Y162 induced strong and specific cellular and humoral immune responses. In vitro study showed that rBCG-EgG1Y162 vaccine not only promote splenocytes proliferation but also active T cell. In addition, the rBCG-EgG1Y162 induced a protection in the mice against secondary infection of Echinococcus granulosus.