In vitro stimulation of neurons by a planar Ti-Au-electrode interface
In vitro stimulation of neurons by a planar Ti-Au-electrode interface
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平面钛金电极界面对神经元的体外刺激
DOI：
10.1063/1.1879109
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
T. Voigt
中科院分区：
文献类型：
--
作者：
A. Reiher;S. Günther;A. Krtschil;H. Witte;A. Krost;T. Opitz;A. D. D. Lima;T. Voigt
我们报告实现一个平面的大面积电极接口，可重复地允许全球的神经元的兴奋和刺激网络活动的产生。界面由两个双指状的钛金电极形成，没有任何隔离涂层，通过电子束蒸发沉积在显微镜盖玻片上。分离的神经细胞从胚胎大鼠大脑皮层上培养这些电极形成电生理活性网络在7天内的文化。这些网络被电激发的电压脉冲的应用，导致在单个神经元的活动或在一个刺激的同步网络活动依赖于脉冲参数。这些参数，如脉冲的数量，脉冲幅度和不同脉冲事件之间的延迟，对刺激成功的影响进行了系统的研究。我们发现阈值为1.8-2.2 V的电压脉冲幅度和电压脉冲持续时间。
We report on the realization of a planar large area electrode interface which reproducibly allows the global excitation of neurons and the generation of stimulated network activity. The interface is formed by two double finger-shaped Ti–Au-electrodes without any isolating coating deposited by electron beam evaporation on microscope cover slips. Dissociated nerve cells from embryonic rat cerebral cortex were cultured on these electrodes forming electrophysiologically active networks within seven days of culture. These networks were electrically excited by application of voltage pulses, resulting either in an activity of single neurons or in a stimulated synchronous network activity in dependence on the pulse parameters. The impact of these parameters, such as the number of pulses, the pulse amplitude and the delay between distinct pulse events, on the stimulation success was systematically investigated. We found threshold values for the voltage pulse amplitude of 1.8–2.2 V and for the voltage pulse duratio...