Feeding behaviour of Glossina pallidipes and G-morsitans centralis on Boran cattle infected with Trypanosoma congolense or T-vivax under laboratory conditions
Feeding behaviour of Glossina pallidipes and G-morsitans centralis on Boran cattle infected with Trypanosoma congolense or T-vivax under laboratory conditions
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DOI：
10.1046/j.1365-2915.2000.00242.x
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发表时间：
2000-09-01
影响因子：
1.9
通讯作者：
Baylis, M
中科院分区：
文献类型：
--
作者：
Moloo, SK;Sabwa, CL;Baylis, M
在实地研究中，采采蝇（双翅目：舌蝇科）捕食感染刚果锥虫（动质体：锥虫科）的牛比捕食感染间日锥虫或未感染的牛更成功。在这里，我们描述了对这种现象的首次实验室研究。在第一个实验中，用刚果锥体克隆 IL 1180 感染的 Boran 公牛和未感染的公牛喂养笼养的 Glossina pallidipes Austen 1 分钟和 5 分钟。在几周内以这种方式记录喂养成功五次。随后在三个附加实验中使用相同的方案，并进行以下组合：G. pallidipes 和感染了间日疟原虫原种 IL 3913 的公牛、G. morsitanscentralis Machado 和感染了 T. congolense 的公牛、以及 G. morsitanscentralis 和感染了间日疟原虫的公牛。这四个实验重复一次，总共做了八个实验。在三项实验中，在一头公牛感染后 1 分钟内测量，采采蝇喂养成功率有所提高（刚果锥虫，两项实验和间日锥虫，一项实验）。对所有数据的综合分析发现，感染前不同组牛的采采蝇饲养成功率没有显着差异，但感染后，受感染牛的采采蝇饲养成功率显着更高（P < 0.001）。刚果锥虫感染比间日锥虫感染更能提高采采蝇的饲养成功率。喂养成功率的增加与贫血水平、皮肤表面温度或寄生虫血症的变化无关。一个可能的解释是锥虫感染对受感染牛的皮肤血管舒张和/或血液凝固的影响。当采食 5 分钟时，几乎所有采采蝇都成功饱食，并且没有发现牛感染对采食成功的影响。
In field studies, tsetse flies (Diptera: Glossinidae) feed more successfully on cattle infected with Trypanosoma congolense Broden (Kinetoplastida: Trypanosomatidae) than on cattle infected with T. vivax Ziemann or uninfected cattle. Here we describe the first laboratory investigation of this phenomenon. In the first experiment, caged Glossina pallidipes Austen were fed for 1 and 5 min on a Boran steer infected with T. congolense clone IL 1180 and on an uninfected steer. Feeding success was recorded in this way five times over several weeks. The same protocol was subsequently used in three additional experiments with the following combinations: G. pallidipes and a steer infected with T. vivax stock IL 3913, G. morsitans centralis Machado and a steer infected with T. congolense, and G. morsitans centralis and a steer infected with T. vivax. The four experiments were replicated once, making eight experiments in total. In three experiments there was increased tsetse feeding success, measured at 1 min, after a steer became infected (T. congolense, two experiments and T. vivax, one experiment). Analysis of all data combined found no significant differences in tsetse feeding success on the different groups of cattle prior to infection, but after infection tsetse feeding success was significantly greater on the infected cattle (P < 0.001). Trypanosoma congolense infection led to a greater increase in tsetse feeding success than T. vivax infection. The increase in feeding success was not related to changes in the level of anaemia, skin surface temperature or parasitaemia. A possible explanation is the effects of trypanosome infection on cutaneous vasodilation and/or blood clotting in infected cattle. When allowed to feed for 5 min, nearly all tsetse engorged successfully and effects of cattle infection on feeding success were not found.