Preferential block of skeletal muscle sodium channels by geographutoxin II, a new peptide toxin from Conus geographus.
Preferential block of skeletal muscle sodium channels by geographutoxin II, a new peptide toxin from Conus geographus.
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Geographutoxin II（一种来自 Conus geographus 的新型肽毒素）优先阻断骨骼肌钠通道。
DOI：
10.1007/bf00580684
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Ohizumi,Y
中科院分区：
文献类型：
--
作者：
Kobayashi,M;Wu,CH;Yoshii,M;Narahashi,T;Nakamura,H;Kobayashi,J;Ohizumi,Y
采用电流钳和电压钳技术研究了地理毒素 II (GTX II)（一种从海洋蜗牛 Conus geographus 中分离出的新型多肽毒素）对神经和肌肉的影响。 GTX II (5×10−7M) 消除了豚鼠骨骼肌的动作电位，但不改变静息电位。然而，即使浓度高于1×10−6M，小龙虾巨轴突、小鼠神经母细胞瘤N1E-115细胞和豚鼠心肌的动作电位也不受GTX II影响。在电压钳制的牛蛙骨骼肌纤维中，钠电流几乎被GTX II (1×10−6M)完全阻断，并在冲洗后缓慢恢复。 GTX II 没有明显改变钠电流的时间进程。这些结果表明，GTX II 选择性阻断骨骼肌钠通道的方式与河豚毒素非常相似。
The effects of geographutoxin II (GTX II), a novel polypeptide toxin isolated from the marine snailConus geographus, on nerves and muscles were studied by current clamp and voltage clamp techniques. GTX II (5×10−7M) abolished the action potential of the guinea pig skeletal muscle without change in the resting potential. However, action potentials of the crayfish giant axon, mouse neuroblastoma N1E-115 cell and guinea pig cardiac muscle were not affected by GTX II even at concentrations higher than 1×10−6M. In the voltage clamped bullfrog skeletal muscle fiber, sodium currents were almost completely blocked by GTX II (1×10−6M), and slowly recovered after washout. The time course of sodium currents was not appreciably altered by GTX II. These results suggest that GTX II selectively blocks skeletal muscle sodium channels in much the same way as tetrodotoxin.