Ultrastructural localization of tartrate-resistant, purple acid phosphatase in rat osteoclasts by histochemistry and immunocytochemistry.
Ultrastructural localization of tartrate-resistant, purple acid phosphatase in rat osteoclasts by histochemistry and immunocytochemistry.
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通过组织化学和免疫细胞化学对大鼠破骨细胞中抗酒石酸盐的紫色酸性磷酸酶进行超微结构定位。
DOI：
10.1002/jbmr.5650040315
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Toverud,SU
中科院分区：
文献类型：
--
作者：
Clark,SA;Ambrose,WW;Anderson,TR;Terrell,RS;Toverud,SU
用纯化的骨源性紫色酸性磷酸酶抗血清，用免疫细胞化学方法检测了抗酒石酸紫色酸性磷酸酶在发育大鼠破骨细胞中的细胞内定位。免疫反应定位结果与以对硝基苯磷酸和10 mM酒石酸盐为底物的酶组织化学结果相比较。两种方法均显示该酶存在于直径达2-3μm的大小不等的囊泡和颗粒中。高尔基体无免疫反应，酒石酸盐完全抑制该细胞器的组织化学活性。没有检测到一致的胞外活性，也没有在褶皱的边缘观察到任何反应产物。破骨细胞中抗酒石酸紫色酸性磷酸酶的定位与该酶的细胞内功能一致。
The intracellular localization of the tartrate‐resistant purple acid phosphatase in osteoclasts of developing rat bone has been determined immunocytochemically using an antiserum to the purified bone‐derived purple acid phosphatase. The localization of the immunoreactivity was compared with the results of enzyme histochemistry usingp‐nitrophenylphosphate as substrate and 10 mM tartrate. Both methods revealed the presence of the enzyme in numerous vesicles of various sizes up to 2–3 μm in diameter and in granules. There was no immunoreactivity in the Golgi apparatus, and tartrate completely inhibited the histochemical activity of this organelle. No consistent extracellular activity could be detected, nor was any reaction product observed at the ruffled border. The localization of the tartrate‐resistant purple acid phosphatase in osteoclasts is consistent with an intracellular function for this enzyme.