Integrative analysis of DNA, macroscopic remains and stable isotopes of dog coprolites to reconstruct community diet.
Integrative analysis of DNA, macroscopic remains and stable isotopes of dog coprolites to reconstruct community diet.
复制标题
狗粪化石的DNA、宏观遗骸和稳定同位素的综合分析，以重建社区饮食。
DOI：
10.1038/s41598-021-82362-6
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发表时间：
2021-02-04
影响因子：
4.6
通讯作者：
Malhi RS
中科院分区：
文献类型：
--
作者：
Witt KE;Yarlagadda K;Allen JM;Bader AC;Simon ML;Kuehn SR;Swanson KS;Cross TL;Hedman KM;Ambrose SH;Malhi RS
古粪石或粪化石经常被用来重建考古遗址的饮食，通常使用宏观分析或靶向DNA扩增和测序。在这里，我们提出了一个综合分析狗粪化石，结合宏观分析，稳定同位素测量，DNA鸟枪测序，检查饮食和健康状况。本研究中使用的犬粪化石是从Janey B中回收的。古德和东圣刘易斯考古遗址，这两个都位于美国底部，一个广泛的密西西比河洪泛平原在伊利诺伊州西南部。根据恢复的背景，粪化石被分配到晚期林地和晚期林地时期（约。公元600-1050年）。鉴于这一时期人类遗骸的稀缺性，这些狗粪化石可以作为了解晚期林地时期人类饮食的替代品。我们发现，晚期林地狗消耗各种鱼类以及鸟类和植物类群，可能包括玉米，也窝藏肠道寄生虫和致病细菌。通过对粪化石的粪便微生物组进行测序，我们发现了与现代狗微生物组的一些相似之处，以及可用于区分现代和古代微生物组的特定类群，不包括土壤污染物。由于狗经常被用作评估人类饮食的替代品，与这些狗一起生活的人可能有类似的饮食，并受到类似的寄生虫影响。这些分析，当整合时，显示了一个更全面的观点古代狗和人类的饮食和健康在该地区的玉米农业最初的扩张比任何单独的方法可以单独。
Paleofeces or coprolites are often used to reconstruct diet at archaeological sites, usually using macroscopic analyses or targeted DNA amplification and sequencing. Here we present an integrative analysis of dog coprolites, combining macroscopic analyses, stable isotope measurements, and DNA shotgun sequencing to examine diet and health status. Dog coprolites used in this study were recovered from the Janey B. Goode and East Saint Louis archaeological sites, both of which are located in the American Bottom, an extensive Mississippi River floodplain in Southwestern Illinois. Based on the context of recovery, coprolites are assigned to the Late Woodland and Terminal Late Woodland periods (ca. 600–1050 AD). Given the scarcity of human remains from this time period, these dog coprolites can be useful as a proxy for understanding human diet during the Late Woodland period. We find that the Late Woodland dogs consumed a variety of fish as well as bird and plant taxa, possibly including maize, and also harbored intestinal parasites and pathogenic bacteria. By sequencing the fecal microbiome of the coprolites, we find some similarities to modern dog microbiomes, as well as specific taxa that can be used to discriminate between modern and ancient microbiomes, excluding soil contaminants. As dogs are often used as a surrogate to assess human diet, humans living with these dogs likely had a similar diet and were affected by similar parasites. These analyses, when integrated, show a more comprehensive view of ancient dog and human diet and health in the region during the initial expansion of maize agriculture than any individual method could alone.
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影响因子：
2.2
作者：
Brugam, R. B.;Little, K.;Martin, T.
通讯作者：
Martin, T.
影响因子：
1.6
作者：
Codron, Daryl;Codron, Jacqui
通讯作者：
Codron, Jacqui
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0.3
作者：
Allitt, Sharon;Stewart, R. Michael;Messner, Timothy
通讯作者：
Messner, Timothy
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1.6
作者：
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通讯作者：
Coltrain, Joan Brenner
影响因子：
1.8
作者：
Ambrose, SH;Buikstra, J;Krueger, HW
通讯作者：
Krueger, HW