Renal sugar transport in the winter flounder. VI. Reabsorption of D-mannose.
Renal sugar transport in the winter flounder. VI. Reabsorption of D-mannose.
复制标题
肾糖运输在冬季鲽鱼中进行。
DOI：
10.1152/ajprenal.1982.242.4.f415
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Kleinzeller,A
中科院分区：
文献类型：
--
作者：
Pritchard,JB;Booz,GW;Kleinzeller,A
采用体内肾清除技术和体外刷边膜（BBM）囊泡技术研究了d -甘露糖（Man）在比目鱼肾脏中的转运。在血浆浓度为50-100 microM Man时，冬季比目鱼（Pseudopleuronectes americanus）可重新吸收高达70%的过滤糖。人的磷酸盐，但不是自由的人，积聚在肾细胞中。苯连菌素、d -葡萄糖、甲基- α -d -葡萄糖苷、甲基- α -d -甘露糖苷和2-脱氧-d -葡萄糖可减少Man的重吸收。在BBM囊泡中，观察到Na+依赖性，根霉素敏感的Man摄取超调（10微米）。Na+依赖性男子摄取是饱和的，表观Km为127微米。冬季比目鱼和南方比目鱼（parichthys letho柱头）的BBM囊泡对人的转运性质相同。通过BBM的顺式抑制和反式刺激实验确定转运特异性。葡萄糖、半乳糖、1,5-无水-d -甘露糖醇（即1-脱氧甘露糖）、2-脱氧-2-氟-d -葡萄糖和甲基- α -甘露糖苷被证明共享载体介导的甘露糖运输。2-脱氧葡萄糖，甲基- α -2-脱氧-d -葡萄糖苷，以及甲基-d -葡萄糖苷的两种异构体（α和β）都没有。相比之下，α -甲基- d -葡萄糖苷抑制体内和BBM囊泡中的d -葡萄糖运输。由此得出结论，Man在比目鱼体内的重吸收是通过Na+-共转运系统进行的，该系统也处理葡萄糖，但与葡萄糖/甲基- α - d -葡萄糖苷重吸收途径不同的是，1)C-1上不需要氧，2)-OH在C-2上的轴向构型（C1构象）很容易被容纳。
The transport of D-mannose (Man) in flounder kidney was studied using renal clearance techniques in vivo and brush border membrane (BBM) vesicles in vitro. At plasma concentrations of 50-100 microM Man, the winter flounder (Pseudopleuronectes americanus) reabsorbed up to 70% of the filtered sugar. Man phosphates, but not free Man, accumulated in renal cells. Reabsorption of Man was reduced by phlorizin, D-glucose, methyl-alpha-D-glucoside, methyl-alpha-D-mannoside, and 2-deoxy-D-glucose. In BBM vesicles a Na+-dependent, phlorizin-sensitive overshoot in Man uptake (10 microM) was seen. Na+-dependent Man uptake was saturable, with an apparent Km of 127 microM. The transport properties for Man were identical in BBM vesicles from the winter flounder and southern flounder (Paralichthys lethostigma). The transport specificity was determined by cis-inhibition and trans-stimulation experiments with BBM. Glucose, galactose, 1,5-anhydro-D-mannitol (i.e., 1-deoxymannose), 2-deoxy-2-fluoro-D-glucose, and methyl-alpha-mannoside were shown to share the carrier-mediating mannose transport. 2-Deoxyglucose, methyl-alpha-2-deoxy-D-glucoside, and both the isomers (alpha and beta) of methyl-D-glucoside did not. In contrast, alpha-methyl-D-glucoside inhibited D-glucose transport both in vivo and in BBM vesicles. It is concluded that Man reabsorption in the flounder occurs via a Na+-cotransport system that also handles glucose but that differs from the glucose/methyl-alpha-D-glucoside reabsorptive pathway in that 1) an oxygen on C-1 is not required, and 2) an axial configuration for -OH on C-2 (C1 conformation) is readily accommodated.
影响因子：
29.4
作者：
Xing, Tong;Zhu, Chenxi;Chen, Lidong
通讯作者：
Chen, Lidong
影响因子：
64.8
作者：
Zhao, Li-Dong;Lo, Shih-Han;Kanatzidis, Mercouri G.
通讯作者：
Kanatzidis, Mercouri G.