Characterization of HIV type 1 genotypes and drug resistance mutations among drug-naive HIV type 1-infected patients in Northern Vietnam.
Characterization of HIV type 1 genotypes and drug resistance mutations among drug-naive HIV type 1-infected patients in Northern Vietnam.
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越南北部未接受药物治疗的 HIV 1 型感染者的 HIV 1 型基因型和耐药突变特征。
DOI：
10.1089/aid.2009.0206
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发表时间：
2010
影响因子：
1.5
通讯作者：
H. Ichimura
中科院分区：
文献类型：
--
作者：
Thi Thu Chung Phan;A. Ishizaki;D. Phung;Xiuqiong Bi;S. Oka;H. Ichimura
为了评估越南北部初治 HIV-1 感染患者中与 HIV-1 耐药相关的突变，我们对 2008 年 206 名（161 名男性和 45 名女性）同意患者收集的样本中的 HIV-1 pol-PR 和 pol-RT 进行了序列分析。从这 206 个样本中，我们成功对 173 个 pol-PR 和 155 个 pol-RT 基因进行了测序。系统发育分析显示，所有患者均感染HIV-1 CRF01_AE。在 3 名患者（1.7%）中发现了主要的蛋白酶抑制剂耐药突变，如 L33F、M46I 和 M46L。 7 名患者 (4.5%) 发现了主要的逆转录酶抑制剂 (RTI) 耐药突变，其中 4 例为单一突变：2 例为 A62V（核苷 RTI 耐药突变），各 1 例为 K103N 和 Y181C（非核苷 RTI 耐药突变）。三名患者有多种 RTI 耐药突变：分别为 2 个、3 个和 7 个。因此，建议在开始抗逆转录病毒治疗 (ART) 之前监测耐药 HIV-1 并进行耐药性测试，以促进越南选择合适的 ART 并获得更好的临床结果。
To evaluate HIV-1 drug resistance-associated mutations among drug-naive HIV-1-infected patients in Northern Vietnam, we performed sequence analysis of HIV-1 pol-PR and pol-RT in samples collected from 206 (161 men and 45 women) consenting patients in 2008. From these 206 samples, we successfully sequenced 173 pol-PR and 155 pol-RT genes. Phylogenetic analysis revealed that all patients were infected with HIV-1 CRF01_AE. Major protease inhibitor resistance mutations, such as L33F, M46I, and M46L, were found in three patients (1.7%). Major reverse-transcriptase inhibitor (RTI) resistance mutations were found in seven patients (4.5%), four of whom had single mutations: A62V (nucleoside RTI resistance mutation) in two cases and K103N and Y181C (nonnucleoside RTI resistance mutation) in one case each. Three patients had multiple RTI resistance mutations: two, three, and seven, respectively. Thus, monitoring for drug-resistant HIV-1 and performing drug resistance testing before initiating antiretroviral therapy (ART) are recommended to facilitate selection of the appropriate ART and better clinical outcomes in Vietnam.