Freeze-fracture study on the erythrocyte membrane during malarial parasite invasion.
Freeze-fracture study on the erythrocyte membrane during malarial parasite invasion.
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DOI：
10.1083/jcb.91.1.55
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发表时间：
1981-10
期刊：
影响因子：
--
通讯作者：
Epstein N
中科院分区：
文献类型：
--
作者：
Aikawa M;Miller LH;Rabbege JR;Epstein N
疟原虫裂殖子侵入红细胞需要在裂殖子和红细胞之间形成连接。在裂殖子进入红细胞内陷的过程中，平行于裂殖子长轴的连接处发生迁移。冰冻断口显示红细胞膜的P面在红细胞膜凹陷的颈部周围有一条窄的菱形排列的颗粒带，E面上有与之匹配的菱形排列的凹坑。这条带对应于在薄片中观察到的红细胞膜和裂殖子膜之间的连接处，可能代表了红细胞内收缩蛋白的锚定位置。红细胞膜P面上的膜内颗粒(IMP)在此连接后消失。当红细胞和细胞松弛素B处理的裂殖子一起孵育时，裂殖子附着在红细胞膜上，并在两者之间形成连接，但侵袭过程不会进一步推进，连接也不会发生移动。虽然没有寄生虫进入，红细胞膜仍然内陷。冷冻断口显示，内陷的红细胞膜的P面几乎没有膜上其他地方发现的IMP，这表明附着过程本身足以产生一个相对不含IMP的双层。
Invasion of erythrocytes by malarial merozoites requires the formation of a junction between the merozoite and the erythrocyte. Migration of the junction parallel to the long axis of the merozoite occurs during the entry of the merozoite into an invagination of the erythrocyte. Freeze-fracture shows a narrow circumferential band of rhomboidally arrayed particles on the P face of the erythrocyte membrane at the neck of the erythrocyte invagination and matching rhomboidally arrayed pits on the E face. The band corresponds to the junction between the erythrocyte and merozoite membranes observed in thin sections and may represent the anchorage sites of the contractile proteins within the erythrocyte. Intramembrane particles (IMP) on the P face of the erythrocyte membrane disappear beyond this junction. When the erythrocytes and cytochalasin B-treated merozoites are incubated together, the merozoite attaches to the erythrocyte membrane and a junction is formed between the two, but the invasion process does not advance further and no movement of the junction occurs. Although there is no entry of the parasite, the erythrocyte membrane still invaginates. Freeze-fracture shows that the P face of the invaginated erythrocyte membrane is almost devoid of the IMP that are found elsewhere on the membrane, suggesting that the attachment process in and of itself is sufficient to create a relatively IMP-free bilayer.