Glucocorticoid implants around the hypothalamic paraventricular nucleus prevent the increase of corticotropin-releasing factor and arginine vasopressin immunostaining induced by adrenalectomy.
Glucocorticoid implants around the hypothalamic paraventricular nucleus prevent the increase of corticotropin-releasing factor and arginine vasopressin immunostaining induced by adrenalectomy.
复制标题
下丘脑室旁核周围的糖皮质激素植入物可防止肾上腺切除术引起的促肾上腺皮质激素释放因子和精氨酸加压素免疫染色的增加。
DOI：
10.1159/000124650
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发表时间：
1986
影响因子：
4.1
通讯作者：
G. Makara
中科院分区：
文献类型：
--
作者：
K. Kovács;J. Kiss;G. Makara
采用糖皮质激素植入和免疫组织化学相结合的方法研究了糖皮质激素对下丘脑促肾上腺皮质激素释放因子（CRF）和精氨酸加压素（AVP）的抑制作用位点。肾上腺切除术增加了室旁核（PVN）前部和内侧小细胞细分中含有 CRF 样免疫反应性的神经元的数量和染色强度，并诱导同一细胞群中 AVP 样免疫反应性的出现。肾上腺切除术的这些效应仅受到靠近 PVN 放置的地塞米松植入物的抑制。单侧PVN植入地塞米松仅在植入侧抑制了肾上腺切除术引起的CRF和AVP免疫染色的变化。植入海马体的地塞米松降低了 PVN 肾上腺切除术的效果，而植入杏仁核和大脑皮层的类似植入物则无效。这些结果表明，糖皮质激素对下丘脑促肾上腺皮质激素分泌的反馈抑制的主要部位是 PVN。
The site of inhibitory action of glucocorticoids on the hypothalamic corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) was studied using a combination of glucocorticoid implantation and immunohistochemistry. Adrenalectomy increased the number and the staining intensity of the neurons containing CRF-like immunoreactivity in the anterior and medial parvicellular subdivisions of the paraventricular nucleus (PVN) and induced the appearance of AVP-like immunoreactivity in the same cell population. These effects of adrenalectomy were inhibited only by those dexamethasone implants which were placed close to the PVN. Unilateral implantation of dexamethasone into the PVN inhibited the adrenalectomy-induced changes in CRF and AVP immunostaining only on the implanted side. Dexamethasone implants placed into the hippocampus decreased the effect of adrenalectomy in the PVN while similar implants into the amygdala and cerebral cortex were ineffective. These results suggest that the primary site of glucocorticoid feedback inhibition on the hypothalamic secretagogues of adrenocorticotropin is the PVN.