Active circulation of bluetongue vaccine virus serotype-2 among unvaccinated cattle in central Italy
Active circulation of bluetongue vaccine virus serotype-2 among unvaccinated cattle in central Italy
复制标题
DOI：
10.1016/j.prevetmed.2004.11.011
复制
发表时间：
2005-05-10
影响因子：
2.6
通讯作者：
Autorino, GL
中科院分区：
文献类型：
--
作者：
Ferrari, G;De Liberato, C;Autorino, GL
2002年在拉齐奥和托斯卡纳（意大利中部）进行蓝舌疫苗接种期间，哨兵牛中发生了几次血清转化，导致怀疑疫苗病毒传播。因此，在2003年，对17个血清转化哨点牛群进行了调查，以了解所涉及病毒的特征。从这些养殖场中，检测了91头未接种动物和57个库蠓样本池中是否存在蓝舌病疫苗病毒（血清型-2）或其他毒株。在17头未接种的哨兵牛和12个拟库蚊或C库的全血中，通过PCR和随后的限制性酶切分析确认了疫苗病毒血清型-2的存在。obsoletus。在17个畜群中，5个畜群仅对疫苗病毒血清型2呈阳性，4个畜群对其他毒株呈阳性，2个畜群对疫苗和其他毒株均呈阳性;其余畜群为病毒学阴性。在疫苗接种运动未包括的地区也检测到了疫苗病毒血清型2。(c)2004 Elsevier B. V.保留所有权利。
Several seroconversions occurring in 2002 among sentinel cattle during the bluetongue-vaccination campaign in Lazio and Tuscany (central Italy) led to the suspicion of vaccine-virus circulation. Therefore in 2003, 17 seroconverting sentinel herds were investigated for the characteristics of the virus involved. From these farms, 91 unvaccinated animals and 57 Culicoides pools were tested for the presence of the bluetongue vaccine virus (serotype-2) or other strains. The presence of vaccine virus serotype-2 was confirmed by PCR followed by restriction analysis in the whole blood of 17 unvaccinated sentinel cattle and 12 pools of Culicoides imicola or C. obsoletus. Of the 17 herds, five were positive only for vaccine virus serotype-2, four were positive for other strains and two for both the vaccine and other strains; the remaining premises were virologicaly negative. The vaccine virus serotype-2 also was detected in areas not included in the vaccination campaign. (c) 2004 Elsevier B.V. All rights reserved.