HIGH-EFFICIENCY PLATING METHOD FOR LEISHMANIA PROMASTIGOTES IN SEMI-DEFINED OR COMPLETELY-DEFINED MEDIUM
HIGH-EFFICIENCY PLATING METHOD FOR LEISHMANIA PROMASTIGOTES IN SEMI-DEFINED OR COMPLETELY-DEFINED MEDIUM
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DOI：
10.2307/3281131
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发表时间：
1983-01-01
影响因子：
1.3
通讯作者：
ULLMAN, B
中科院分区：
文献类型：
--
作者：
IOVANNISCI, DM;ULLMAN, B
一种简单的技术是为分离来自杜氏乳杆菌和热带乳杆菌的单个前鞭毛体细胞的克隆而开发的，涉及使用半固体琼脂。两种利什曼原虫前鞭毛体在含有 10% 胎牛血清的半限定培养基或缺乏血清的完全限定培养基中均高效形成离散菌落。在含有 10% 胎牛血清的生长培养基中 8-14 天出现可见菌落。用牛血清白蛋白和Tween-80替代胎牛血清使集落形成时间增加50%，但不影响克隆效率。从半固体琼脂转移至液体悬浮培养物的菌落的存活率为100%。
A simple technique, developed for the isolation of clones derived from single, promastigote cells of L. donovani and L. tropica, involved the use of semisolid agar. Both species of Leishmania promastigotes formed discrete colonies at high efficiency either in semidefined medium containing 10% fetal calf serum or in completely-defined medium lacking serum. Visible colonies appeared between 8-14 days in growth medium containing 10% fetal calf serum. Replacement of the fetal calf serum with bovine serum albumin and Tween-80 increased the time of colony formation by 50% but did not affect the cloning efficiency. Viability of colonies transferred from semisolid agar to liquid suspension culture was 100%.