Respiratory control by ventral surface chemoreceptor neurons in rats
Respiratory control by ventral surface chemoreceptor neurons in rats
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DOI：
10.1038/nn1357
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发表时间：
2004-12-01
影响因子：
25
通讯作者：
Guyenet, PG
中科院分区：
文献类型：
--
作者：
Mulkey, DK;Stornetta, RL;Guyenet, PG
一个长期存在的理论认为，中枢化学感受，CO2检测和呼吸调节的CNS机制，涉及位于延髓腹侧表面（VMS）的神经元。使用在体内和体外的电生理记录，我们确定VMS神经元内的大鼠后斜方核（RTN），具有适合这些难以捉摸的化学感受器的特性。这些多巴胺能神经元在体内被CO2强烈激活，而多巴胺能神经元则不然。他们的CO2敏感性不受呼吸模式发生器的药物阻断的影响，并且在没有颈动脉体输入的情况下持续存在。RTN的CO2敏感神经元有广泛的树突沿着VMS和他们支配的关键pontomedullary呼吸中心。在脑干切片中，具有明显相似形态的RTN神经元的子集被酸化和CO2强烈激活。它们的pH敏感性是内在的，涉及背景K+电流。简而言之，RTN的CO2敏感神经元是长期寻求的VMS化学感受器的良好候选者。
A long-standing theory posits that central chemoreception, the CNS mechanism for CO2 detection and regulation of breathing, involves neurons located at the ventral surface of the medulla oblongata (VMS). Using in vivo and in vitro electrophysiological recordings, we identify VMS neurons within the rat retrotrapezoid nucleus (RTN) that have characteristics befitting these elusive chemoreceptors. These glutamatergic neurons are vigorously activated by CO2 in vivo, whereas serotonergic neurons are not. Their CO2 sensitivity is unaffected by pharmacological blockade of the respiratory pattern generator and persists without carotid body input. RTN CO2-sensitive neurons have extensive dendrites along the VMS and they innervate key pontomedullary respiratory centers. In brainstem slices, a subset of RTN neurons with markedly similar morphology is robustly activated by acidification and CO2. Their pH sensitivity is intrinsic and involves a background K+ current. In short, the CO2-sensitive neurons of the RTN are good candidates for the long sought-after VMS chemoreceptors.