DOPAMINE PRODUCTION BY ISOLATED GLOMERULI AND TUBULES FROM RAT KIDNEYS
DOPAMINE PRODUCTION BY ISOLATED GLOMERULI AND TUBULES FROM RAT KIDNEYS
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DOI：
10.1139/y85-027
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发表时间：
1985-01-01
影响因子：
2.1
通讯作者：
HATCHER, C
中科院分区：
文献类型：
--
作者：
BAINES, AD;DRANGOVA, R;HATCHER, C
To locate the sites of dopamine (D) production in rat renal cortex, glomeruli and proximal tubules were separated by sieving or centrifugation in Percoll after collagenase digestion. After centrifugation layer I contained 60-80% glomeruli and 20-40% tubule fragments, half of which did not stain with alkaline phosphatase, layer II contained 0-5% glomeruli, 10-25% tubule fragments other than proximal tubules and 70-85% proximal tubule fragments. Layer IV contained 85-95% proximal tubules. Gluconeogenic rates were (in .mu.mol/h/g wet weight) as follows: I, 4 .+-. 1; II, 7 .+-. 2; and IV, 16 .+-. 1. Norepinephrine (NE) content was (pmol/g wet weight) I, 310 .+-. 30; II, 540 .+-. 40; IV, 195 .+-. 60. D content was (pmol/g wet weight) I, 26 .+-. 6; II, 46 .+-. 13; IV, 33 .+-. 7. Surgical denervation 4-6 days previously reduced the NE content of layers I and II to 35 .+-. 10 (P < 0.001) and of IV to 60 .+-. 20 (P < 0.05) and the D content of layers I and II to 13 .+-. 6 and 6 .+-. 6 pmol/g, respectively (P < 0.01); D content of layer IV was unchanged. D production from 10-7 M 3,4-dopa was (nmol/g/min) I, 0.2 .+-. 0.03; II, 0.7 .+-. 0.1; IV, 1.0 .+-. 0.04. Dopa consumption was (nmol/g/min) I, 0.6 .+-. 0.1; II, 1.4 .+-. 0.3; and IV, 1.8 .+-. 0.2. Denervation did not change D production or dopa consumption. Glomeruli without arterioles or tubules, obtained by sieving, contained no D or NE and did not produce D from dopa. Proximal tubules produced at least 5 times more D from dopa than glomeruli and distal tubules. Renal nerves did not contribute significantly to D production. There was no evidence of specific D-containing nerves associated with rat glomeruli.
