CE-LIF method for the separation of anthracyclines: application to protein binding analysis in plasma using ultrafiltration.
CE-LIF method for the separation of anthracyclines: application to protein binding analysis in plasma using ultrafiltration.
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DOI：
10.1002/jssc.200700629
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发表时间：
2008-06
影响因子：
3.1
通讯作者：
Gillian Whitaker;Amy Lillquist;Stephanie A. Pasas;R. O'Connor;F. Regan;C. Lunte;M. Smyth
中科院分区：
文献类型：
--
作者：
Gillian Whitaker;Amy Lillquist;Stephanie A. Pasas;R. O'Connor;F. Regan;C. Lunte;M. Smyth
蒽环类药物是广泛用于治疗肺癌和卵巢癌等癌症的化疗药物。使用与 LIF 耦合的 CE 实现了蒽环类药物、柔红霉素、阿霉素和表柔比星的同时测定，激发和发射波长分别为 488 和 560 nm。使用硼酸盐缓冲液（105 mM，pH 9.0）和 30% MeOH，获得了三种蒽环类药物的稳定且可重复的分离。所开发的方法被证明能够监测蒽环类药物的治疗浓度（50-50 000 ng/mL）。根据 S/N = 3 计算，LOD 达到 10 ng/mL。使用开发的 CE 方法，通过超滤测量体外蛋白质与血浆的结合，根据这项研究，估计的蛋白质结合确定在 77-94% 的范围内。
Anthracyclines are chemotherapeutic drugs that are widely used in the treatment of cancers such as lung and ovarian cancers. The simultaneous determination of the anthracyclines, daunorubicin, doxorubicin and epirubicin, was achieved using CE coupled to LIF, with an excitation and emission wavelength of 488 and 560 nm, respectively. Using a borate buffer (105 mM, pH 9.0) and 30% MeOH, a stable and reproducible separation of the three anthracyclines was obtained. The method developed was shown to be capable of monitoring the therapeutic concentrations (50-50 000 ng/mL) of anthracyclines. LODs of 10 ng/mL, calculated at an S/N = 3, were achieved. Using the CE method developed, the in vitro protein binding to plasma was measured by ultrafiltration, and from this investigation the estimated protein binding was determined to be in the range of 77-94%.