Delineation of a core RNA element required for Kaposi's sarcoma-associated herpesvirus ORF57 binding and activity.
Delineation of a core RNA element required for Kaposi's sarcoma-associated herpesvirus ORF57 binding and activity.
复制标题
描述卡波西肉瘤相关疱疹病毒 ORF57 结合和活性所需的核心 RNA 元件。
DOI：
10.1016/j.virol.2011.08.006
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Conrad,NicholasK
中科院分区：
文献类型：
--
作者：
Sei,Emi;Conrad,NicholasK
The Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 protein is an essential multifunctional regulator of gene expression. ORF57 interaction with RNA is necessary for ORF57-mediated posttranscriptional functions, but little is known about the RNA elements that drive ORF57-RNA specificity. Here, we investigate the cis-acting factors on the KSHV PAN RNA that dictate ORF57 binding and activity. We show that ORF57 binds directly to the 5′ end of PAN RNA in KSHV-infected cells. Furthermore, we employ in vitro and cell-based assays to define a 30-nucleotide (nt) core ORF57-responsive element (ORE) that is necessary and sufficient for ORF57 binding and activity. Mutational analysis of the core ORE further suggests that a 9-nt sequence is a specific binding site for ORF57. These studies provide insight into ORF57 specificity determinants and lay a foundation for future analyses of cellular and viral ORF57 targets.
